乙醇酸氧化酶和模式生物含有富苯丙氨酸的碱性蛋白  

PHE-RICH BASIC PROTEIN EXISTED IN GLYCOLATE OXIDASE AND IN SEVERAL MODEL ORGANISMS

作　　者：曾广翔[1] 陈佳[1] 陈雪敏[1] 黄颖沂[1] 谢莉[1] 林子衍[1] 陈梓谦[1] 徐杰[1] 

机构地区：[1]华南师范大学生命科学学院,广东省植物发育生物工程重点实验室,广东广州510631

出　　处：《华南师范大学学报（自然科学版）》2010年第4期93-97,共5页Journal of South China Normal University(Natural Science Edition)

基　　金：广东省高等教育本科教学改革项目(BKJGYB2008042)

摘　　要：作者在之前研究中已证实提取的乙醇酸氧化酶(GO)实际上是GO和富phe碱性蛋白(BP)的混合物,BP因在SDS-PAGE中向负极泳动而容易被忽略.该文再次提取比活为113.4 U/(min.mg)的GO和BP的混合物.该混合物呈现phe的258 nm特征吸收峰,表明BP可能位于GO外部.类似的BP也可能存在于拟南芥、水稻等模式植物和大肠杆菌的粗蛋白中.In previous study,we had proved that extracted glycolate oxidase（GO） was actually a mixture GO and phe-rich basic protein（BP）,and the BP was ignored easily as it migrated towards the cathode in SDS-electrophoresis.In this paper,the mixture of GO and BP with a specific activity of 113.4 U/（min·mg） was extracted.The UV spectrum of the mixture showed an absorption peak at 258 nm which is the characteristic adsorption of phenylalanine,suggesting that BP located on the surface of GO.Similar BP also existed in crude protein extracts from several model species of organism,including Arabidopsis thaliana,Oryza sativa and Escherichia coli.

关 键 词：乙醇酸氧化酶 富phe碱性蛋白 SDS-电泳 负极泳动 

分 类 号：Q51[生物学—生物化学]