Metamorphosis of Magnetospirillum magneticum AMB-1 cells  

作　　者：张风丽 张葵 赵三军 肖天 Michel DENIS 吴龙飞 

机构地区：[1]Laboratoire de Chimie Bactérienne, UPR9043, Institut de Biologie Structurale et Microbiologie [2]Laboratory of Marine Biotechnology, School of Life Sciences & Biotechnology, Key Laboratory of Microbial Metabolism, Ministry of Education, Shanghai Jiao Tong University [3]Laboratoire de Microscopies et d’Etude de Nanostructures (LMEN), Université de Reims [4]Key Laboratory of Marine Ecology & Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences [5]Laboratoire de Microbiologie, Géochimie et Ecologie Marines, CNRS UMR6117, Centre d'Océanologie de Marseille, Université de la Méditerranée

出　　处：《Chinese Journal of Oceanology and Limnology》2010年第2期304-309,共6页中国海洋湖沼学报（英文版）

基　　金：Supported by the CAS/SAFEA International Partnership Program for Creative Research Teams (Research and Applications of Marine Functional Genomics);the Haiwaijie chuxuezhe-Fund of the Chinese Academy of Sciences (2006-1-15);the fund from MATHAB (No. MH200804);a CNRS scholarship for FZ, and National Natural Science Foundation of China (No. 40776094)

摘　　要：Magnetospirillum magneticum strain AMB-1 belongs to the family of magnetotactic bacteria. It possesses a magnetosome chain aligning, with the assistance of cytoskeleton filaments MamK, along the long axis of the spiral cells. Most fresh M. magneticum AMB-1 cells exhibit spiral morphology. In addition, other cell shapes such as curved and spherical were also observed in this organism. Interestingly, the spherical cell shape increased steadily with prolonged incubation time. As the actin-like cytoskeleton protein MreB is involved in maintenance of cell shapes in rod-shaped bacteria such as Escherichia coli and Bacillus subtilis, the correlation between MreB protein levels and cell shape was investigated in this study. Immunoblotting analysis showed that the quantity of MreB decreased when the cell shape changed along with incubation time. As an internal control, the quantity of MamA was not obviously changed under the same conditions. Cell shape directs cell-wall synthesis during growth and division. MreB is required for maintaining the cell shape. Thus, MreB might play an essential role in maintaining the spiral shape of M. magneticum AMB-1 cells.Magnetospirillum magneticum strain AMB-1 belongs to the family of magnetotactic bacteria. It possesses a magnetosome chain aligning, with the assistance of cytoskeleton filaments MamK, along the long axis of the spiral cells. Most fresh M. magneticum AMB-1 cells exhibit spiral morphology. In addition, other cell shapes such as curved and spherical were also observed in this organism. Interestingly, the spherical cell shape increased steadily with prolonged incubation time. As the actin-like cytoskeleton protein MreB is involved in maintenance of cell shapes in rod-shaped bacteria such as Escherichia coli and Bacillus subtilis, the correlation between MreB protein levels and cell shape was investigated in this study. Immunoblotting analysis showed that the quantity of MreB decreased when the cell shape changed along with incubation time. As an internal control, the quantity of MamA was not obviously changed under the same conditions. Cell shape directs cell-wall synthesis during growth and division. MreB is required for maintaining the cell shape. Thus, MreB might play an essential role in maintaining the spiral shape ofM. magneticum AMB-1 cells.

关 键 词：Magnetotactic bacteria Magnetospirillum magneticum AMB-1 MREB 

分 类 号：Q943[生物学—植物学]