Molecular Scrippsiella phylogenetic analysis of dinoflagellate trochoidea isolated from the East Asianwaters  

作　　者：TANG Xianghai YURencheng ZHANG Qingchun WANG Yunfeng YAN Tian ZHOU Mingjiang 

机构地区：[1]Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China [2]Graduate School of Chinese Academy of Sciences, Beijing 100039, China

出　　处：《Chinese Journal of Oceanology and Limnology》2010年第2期323-328,共6页中国海洋湖沼学报（英文版）

基　　金：Supported by the Knowledge Innovation Program of Chinese Academy of Sciences （No. KZCX2-YW-208） and National Natural Science Foundation of China （No. 40676072）

摘　　要：Previous studies found intraspecific diversity in Scrippsiella trochoidea A. R. Loeblich Ill, a widely distributed calcareous cyst-producing dinoflagellate. In this study, three strains （ST-I, ST-D6 and ST-K） of S. trochoidea isolated from the East Asian waters were studied, together with other geographical strains, to resolve their phylogenetic relationships. For the three East Asian isolates, two highly diverse regions of nuclear-encoded ribosomal DNA （rDNA）, the 5.8S rDNA and its flanking internal transcribed spacers 1 and 2, and the 5＇ portion of the large-subunit rDNA （encompassing the ＂DI＂ and ＂D2＂ domains） were sequenced. Homologous sequences from other geographical isolates were selected from the GenBank database and the phylogenetic relationships were inferred from the molecular data of these strains. Strains of S. trochoidea were found to cluster into three major clades （STR1, STR2 and STR3）, as reported in previous studies. Two of the three strains ST-1 and ST-K, were grouped in clade STR2, the other strain, ST-D6, belonged to clade STR3. The intraspecific diversity ofS. trochoidea in East Asian waters makes it necessary to carry out phylogenetic investigations in combination with data of biogeography, population dynamics, and life cycle on the ecophysiology of a region.以前的研究在 Scrippsiella trochoidea A. R 发现 intraspecific 差异。Loeblich III，广泛地分布式的石灰质的生产包囊的 dinoflagellate。在这研究， S 的三紧张(ST-1， ST-D6 和 ST-K ) 。从东方亚洲水孤立的 trochoidea 被学习，和另外的地理紧张，到解决他们的种系发生的关系。因为三个东方亚洲人孤立，原子编码 ribosomal DNA (rDNA ) 的二个高度多样的区域， 5.8S rDNA 和它的 flanking 内部抄录分隔符 1 和 2，并且大子单元的 rDNA 的 5 鈥 ? 部分(包含鈥淒1 鈥 ? 和鈥淒2 鈥 ? 领域) 被定序。从其它的相应序列地理孤立从 GenBank 数据库被选择，种系发生的关系从这些紧张的分子的数据被推断。S 的紧张。trochoidea 被发现聚类进三主要 clades (STR1， STR2 和 STR3 ) ，在以前的研究报导了。三中的二个拉紧 ST-1 和 ST-K，在 clade STR2 被组织，另外的紧张， ST-D6，属于 clade STR3。S 的 intraspecific 差异。东方亚洲水里的 trochoidea 使在一个区域的生态生理学上与生物地理学，人口动力学，和生命周期的数据在联合执行种系发生的调查必要。关键词 Scrippsiella trochoidea - 发展史 - rDNA - 分子的 systematics 由中国科学院的知识革新节目支持了(没有。KZCX2-YW-208 ) 并且中国(号码 40676072 )

关 键 词：Serippsiella trochoidea PHYLOGENY RDNA molecular systematics 

分 类 号：S852.659.5[农业科学—基础兽医学] X55[农业科学—兽医学]