一株高温解烃菌的筛选及性能评价  

Screening of a strain for hydrocarbon degrading and evaluation of its performance

作　　者：何新[1] 何翠香[2] 徐登霆[1] 陈连喜[1] 陈新浩[1] 吴涛 

机构地区：[1]胜利油田分公司采油工艺研究院,山东东营257000 [2]胜利油田分公司地质科学研究院,山东东营257000 [3]中国石油测井有限公司长庆事业部解释中心,陕西西安710201

出　　处：《西安石油大学学报（自然科学版）》2010年第6期73-75,共3页Journal of Xi’an Shiyou University（Natural Science Edition）

基　　金："十一五"国家科技支撑计划项目"油田采油综合节水技术开发"(编号:2006BAB04B02);中石化前瞻性项目"油藏驱油微生物群落调控及变化规律研究"(编号:YKB0707)资助

摘　　要：从油田产出水中筛选出一株能在高温条件下以原油为碳源的烃类降解菌D-1,通过形态、生理生化特征分析,确定该菌株为芽孢杆菌属.测定了pH、温度和盐度对降解能力的影响,确定了最佳生长条件,并用该菌株进行了物模驱油实验.实验结果表明:该菌最佳pH和温度分别为6.0和60℃;其中盐度为0.2%时,降解率达到64.3%,对盐离子具有较好的耐受性;在物理模拟驱油实验中培养10 d后可提高原油采收率5.6%.菌株D-1在微生物采油中具有很好的应用前景.A strain D-1 for hydrocarbon degrading is separated from oilfield produced water.It is identified as Bacillus according to the analyses of its morphology and physiological and biochemical features.The effects of pH value,temperature and salinity on its degradation performance and its optimum growing conditions are determined.The oil displacement experiment of the strain is carried out with a physical model.It is shown that the degradation rate of the strain to hydrocarbons can reach to 64.3% when pH value is 6,temperature is 60 ℃,and the salinity is 0.2%.The strain has good tolerance to salt ions.The oil displacement experiment result of the strain also shows that the recovery factor of crude oil can be increased by 5.6% after the train is trained 10 days.Therefore,the strain has a good application prospects in microbial oil recovery.

关 键 词：高温解烃菌 降解性能 降解条件 物模驱油 

分 类 号：TE39[石油与天然气工程—油气田开发工程]