HPLC-MS/MS法同时测定人血浆和尿液中哌拉西林他唑巴坦的浓度  

Simultaneous determination of piperacillin and tazobactam in human plasma and urine by HPLC-MS/MS

作　　者：冯金泉[1] 吴华[1] 冯文利[1] 张佳丽[1] 王华光[1] 潘世芬[1] 王鹤尧[1] 张征[1] 

机构地区：[1]首都医科大学附属北京朝阳医院药事部,北京100020

出　　处：《中国药物应用与监测》2010年第3期144-147,共4页Chinese Journal of Drug Application and Monitoring

摘　　要：目的:建立同时测定人血浆和尿液中哌拉西林他唑巴坦浓度的高效液相色谱-质谱联用方法。方法:采用Waters C18柱(4.6mm×50mm,5μm),流动相为乙腈-0.5%甲酸,兰索拉唑为内标,以多反应监测(MRM)扫描方式进行检测,监测离子质荷比哌拉西林m/z516.2→233.1,他唑巴坦m/z299.0→138.1,兰索拉唑m/z368.1→163.9。血浆样品经乙腈沉淀蛋白后取上清液进样,尿液样品稀释后经乙腈沉淀蛋白进样。结果:血浆和尿液中哌拉西林、他唑巴坦的线性范围均为0.10～100.00μg·mL-1,低、中、高3个浓度日内、日间精密度均小于10%。结论:本方法灵敏度高,重现性好,操作简便快捷,可用于生物样本中哌拉西林、他唑巴坦钠的浓度测定。Objective：The HPLC-MS/MS method was established for the simultaneous determination of piperacillin and tazobactam in human plasma and urine.Methods：A Waters C18 column（4.6 mm×50 mm,5 μm） was used with the mobile phase of acetonitrile and 0.5% formic acid.Lansoprazole was used as internal standard.Multiple reaction monitoring（MRM） mode was employed,and the transition of m/z was 516.2→233.1 for piperacillin,299.0→138.1 for tazobactam and 368.1→163.9 for lansoprazole.Piperacillin and tazobactam were separated from plasma and diluted urine by protein precipitation with acetonitrile.Results：The calibration curves of piperacillin and tazobactam were linear in the range of 0.10 ～ 100.00 μg·mL-1.RSDs of the interday and intra-day precisions of low,medium and high concentrations were all less than 10%.Conclusion：The method is sensitive,specific,repeatable and accurate for the determination of piperacillin and tazobactam in human plasma and urine.

关 键 词：哌拉西林 他唑巴坦 高效液相色谱-质谱联用 血浆 尿液 

分 类 号：R96[医药卫生—药理学]