甜菜夜蛾增殖马尾松毛虫质型多角体病毒研究  

Propagation of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus in Substitutive Host Spodoptera exigua

作　　者：肖宇宙[1,2] 孙修炼[1] 汤显春[1] 彭辉银[1] 

机构地区：[1]病毒学国家重点实验室,中国科学院武汉病毒研究所,武汉430071 [2]中国科学院研究生院,北京100049

出　　处：《应用与环境生物学报》2010年第1期84-90,共7页Chinese Journal of Applied and Environmental Biology

基　　金：中国科学院农业扶贫专项项目(No.KSCX2-YW-N-011)~~

摘　　要：以甜菜夜蛾(Spodoptera exigua)作为替代宿主增殖马尾松毛虫质型多角体病毒(DpCPV)的研究结果表明,DpCPV在甜菜夜蛾体内连续传代3次后得到的DpCPV-Se3与DpCPV具有相同的电泳图谱;多角体和病毒粒子在电镜下观察,未发现形态上的明显变化.生测结果表明,DpCPV-Se3与DpCPV回接松毛虫试验,其半数致死浓度分别为0.92×103 PIB/mL和1.44×103PIB/mL,二者毒力差异不显著.以甜菜夜蛾增殖DpCPV,多角体产量达到2.34×108PIB/头.用DpCPV-Se3和DpCPV的S7和S10片段分别进行同源性序列分析,结果没有明显变化.认为利用甜菜夜蛾增殖DpCPV用于生物防治具有可行性.The infectivity and genetic stability of Dendrolimus punctatus cytoplasmic polyhedrosis virus（DpCPV） propagated in the substitutive host Spodoptera exigua were investigated.DpCPV-Se3,which was propagated in S.exigua for three passages,had identical electrophoresis pattern with the original isolate.They were shown with no differences in polyhedra and virion morphology under electron microscope.According the results of bioassays,DpCPV-Se3 had a similar infectivity with original isolate against the second instars of D.punctatus and the median lethal concentration（LC50） of DpCPV-Se3（0.92× 103 PIB/mL） was not significantly different from that of the original isolate（1.44×103 PIB/mL）.Sequencing analysis indicated that the variations of S7 and S10 fragments between DpCPV-Se3 and DpCPV were minor,and S.exigua might be a good substitutive host for the mass-production of DpCPV for biological control.

关 键 词：马尾松毛虫 质型多角体病毒 甜菜夜蛾 替代宿主 

分 类 号：S476.13[农业科学—农业昆虫与害虫防治]