满山红油液相色谱指纹特征的分析  

Fingerprint Analysis of Farrerol Rhododendri Daurici Liquid Chromatogram

作　　者：方洪壮[1] 李百强[2] 方芳[1] 崔晓彪 高旭光[2] 

机构地区：[1]佳木斯大学,黑龙江佳木斯154007 [2]黑龙江省药品不良反应监测中心,黑龙江哈尔滨150076 [3]黑龙江龙桂制药有限公司,黑龙江绥化152051

出　　处：《中医药信息》2010年第6期68-70,共3页Information on Traditional Chinese Medicine

基　　金：黑龙江省中医药重点科研项目(ZHY06-Z43)

摘　　要：目的:比较分析满山红油HPLC指纹特征,为满山红油的质量控制提供基础性研究资料。方法:采用Hypersil C18色谱柱,以乙腈-0.1%磷酸水溶液为流动相进行梯度洗脱,流速:1.0mL.min-1,检测波长:210nm,测定了19批不同来源满山红油的HPLC-UV指纹图谱。用化学计量学的聚类分析和主成分分析方法结合相似度,比较分析全息指纹特征。结果:不同批次的满山红油按其指纹特征可分成两类。结论:牻牛儿酮峰对满山红油指纹特征类别的划分与指纹特征的相关性影响极大。Objective： Comparative analysis of chromatographic fingerprint of Farrerol Rhododendri daurici in order to provide basis research material for the quality control of the volatile oil.Methods： 19 batches of samples were detected at 210 nm and separated on a Hypersil C18 column with a gradient elution of acetonitrile and 0.1% aqueous phosphoric acid at a flow rate of 1.01mL.min-1.The multi-data fingerprint was analyzed by the two Chemometrics methods,cluster analysis and principal components analysis,combined with similarity.Result： The study showed that the samples can be divided to into two classes according to the HPLC profiles.Conclusion： The classification and similarity of chromatographic fingerprint were obviously affected by the germacrone peak in the chromatogram of Farrerol Rhododendri daurici.

关 键 词：满山红油 指纹图谱 化学计量学 牻牛儿酮 

分 类 号：R284.1[医药卫生—中药学]