利用SRAP分子标记分析结球大白菜的遗传多样性  

Analysis of Genetic Diversity of Chinese Cabbage by SRAP Markers

作　　者：徐立功[1] 韩太利 

机构地区：[1]山东省潍坊市农业科学院,山东潍坊261041

出　　处：《中国园艺文摘》2010年第12期1-4,共4页Chinese Horticulture Abstracts

基　　金：国家农业科技成果转化资金项目(2007GB2C600181);国家科技支撑计划(2008BADA6B05);国家现代农业产业技术体系项目

摘　　要：利用SRAP技术对各种形态具有代表性的46个结球大白菜骨干自交系进行了DNA多态性分析。从170对引物中筛选出8对引物应用于扩增基因组DNA,共获得328条重复性好、清晰可辨的条带,大小在200～2500bp之间,其中283条为多态性带(86.28%)。其平均多样性指数为0.3840,每个位点的有效等位基因数是1.4170,遗传相异性系数分布在0.050～0.7553之间,平均0.5508,标准差为9.47×10-2。利用UPGMA方法聚类分析,可将试材分成五大类群,与传统的形态学分类有一定差异。第一、二、三类群为高桩材料,第四类群仅包括VB06-18,第五类群囊括绝大多数的矮桩材料。第五类群又明显分成两个亚群,春材料集中分布在一亚群之中。结果显示:结球大白菜自交系遗传背景复杂,类群内相似性程度较高。今后需加大国外资源引进力度,深入挖掘原始地方品种,才能使大白菜育种工作取得突破性进展。同时,该试验研究结果对大白菜的杂交育种工作具有一定的指导作用。The SRAP analysis was conducted in order to investigate the blood relationship among the 46 Chinese cabbage inbred lines. 8 primer pairs were selected from 170 primer pairs to amplify the genomic DNA. Totalling 328 SRAP fragments between 200 bp and 2 500 bp were obtained, and 283 of them were polymorphic markers. The polymorphic fragment percentage was 86.28%. The polymorphism information content was 0.3840. The effective number of alleles was 1.4170. The genetic dissimilarity of 46 Chinese cabbages was from 0.050 to 0.7553, and the average was 0.5508. Cluster analysis using UPGMA method showed that 46 inbred lines could be divided into five main groups. Three of them were high stake type; VB06-18 was a single group, and most short stake type belonged to the fifth group. The fifth group could also be divided into two sub-groups and spring materials were classified into one sub-group. The results showed the genetic background of heading Chinese cabbage was complex and the similarity of different materials in one group was high. Only by importing foreign germplasm resources at full blast and fully utilize primitive local variety, a breakthrough in Chinese cabbage breeding could be made. And the study results of this experiment had a certain role in guiding the work of cross-breeding of Chinese cabbage.

关 键 词：结球大白菜 SRAP标记 自交系 遗传多样性 

分 类 号：S634.1[农业科学—蔬菜学]