不同方法提取天麻DNA的研究  

Study on Different Methods extracting the DNA of Gastrodia elata Blume

作　　者：王晓玲[1] 王晓多 常楚瑞[3] 王晓丽[3] 

机构地区：[1]贵州大学植保系,贵州贵阳550025 [2]贵阳市花溪区果树站,贵州贵阳550025 [3]贵阳医学院药用植物与生药学教研室,贵州贵阳550004

出　　处：《安徽农业科学》2010年第31期17389-17390,共2页Journal of Anhui Agricultural Sciences

基　　金：科技部科技攻关计划(2004BA721A33);贵州省中药现代化科技产业研究开发专项[黔科合中药专字(2003)51];贵州省科技攻关计计划[黔科合农社字(2001)1125]

摘　　要：[目的]探索适合于天麻DNA的提取方法。[方法]采用3种方法提取天麻DNA,测定提取的天麻DNA纯度与产量,并对其扩增产物进行电泳分析。[结果]改进的CTAB法提取的天麻DNA产量和纯度均较高,扩增产物的电泳条带也较明显。[结论]改进的CTAB法较适合用于天麻DNA的提取。[ Objective ] The aim was to explore the suitable extraction method for DNA from Gastrodia elata Blume. [ Method] Three extraction ＂methods were used to extract the Gastrodia elata Blume DNA,the yield and purity of DNA were determined,then the amplified products were analyzed by electrophoresis. [ResuhJ The yield＇and purity of DNA extracted from Gastrodia elata Blume with improved CTAB method were highest, and the electrophoretic bands of amplified products were obvious. [ Conclusion ] The improved CTAB method is suitable for extracting the DNA from Gastrodia elata Blume.

关 键 词：天麻 DNA提取方法 PCR分析 

分 类 号：S567.9[农业科学—中草药栽培]