不同生态型五节芒对Cd胁迫的生理响应  

Physiological Responses of Different Ecotypes of Miscanthus floridulus under Cd Contamination

作　　者：谢国晖 秦建桥[2,3] 赵鹏[3] 赵华荣[3] 

机构地区：[1]广东省佛山市高明区环境保护监测站,广东佛山528500 [2]广东省环境科学研究院,广东广州510045 [3]中山大学环境科学与工程学院,广东广州510275

出　　处：《安徽农业科学》2010年第31期17504-17507,共4页Journal of Anhui Agricultural Sciences

基　　金：广东省自然科学基金团队项目(06202438);中山大学985工程环境污染控制与修复技术创新平台项目

摘　　要：[目的]揭示不同生态型五节芒在抵抗Cd胁迫机制方面的异同。[方法]以粤北大宝山矿山开采地优势植物五节芒为试验材料，比较了五节芒非矿山生态型（采自惠州市博罗县丘陵山地）和矿山生态型（采自大宝山矿区）在相同Cd处理浓度梯度下的生理响应。[结果]2种生态型五节芒叶片中叶绿素a、叶绿素b以及叶绿素总量都与Cd胁迫浓度成极显著负相关（P〈0．01），但矿山型下降的程度低于非矿山型，2种生态型五节芒叶片的叶绿素a／b值都随cd处理浓度的增加而增大，说明Cd对五节芒叶绿素b的伤害要大于叶绿素a。2种生态型五节芒叶片中可溶性蛋白的含量随Cd处理浓度的增加均呈先下降后上升的趋势，均在20mg／kg处理处达到最低。随Cd处理浓度的增加，非矿山型五节芒叶片中可溶性糖含量先下降再升高，浓度在10～80mg/kg处理组均显著低于对照（P〈0．05），矿山型五节芒叶片中可溶性糖含量随Cd处理浓度增加先升高再下降，2种生态型五节芒叶片中可溶性糖含量随Cd处理浓度增加响应有所不同。[结论]矿山型五节芒的耐重金属Cd要强于非矿山型。[ Objective ] The aim was to reveal the different principles of two ecotypes of Miscanthus floridulus to resistant Cd stress. [ Method ] With the advantageous plant of Miscanthus floridulus in Baoshan mining area as the experiment material, the different physiological responses of the non-mining ecotype of Miscanthus floridulus （collected from hills of Huizhou Boluo） and the mining ecotype of Miscanthus floridulus （collected from the Dabaoshan mine） under the same Cd concentration treatment were compared. [ Result ] The chlorophyll a, chlorophyll b and to- tal chlorophyll in both two ecotypes of Miscanthus floridulus had an extremely significant negative correlation with the Cd treatment concentration（ P 〈 0.01 ）, but the decrease level was lower in the mining ecotype than in the non-mining ecotype. The ratio of chlorophyll a/b of two ecotypes all increased with the increase＇of the Cd treatment concentration,which indicated that there was more serious damage to chlorophyll b than chlorophyll a by Cd. The dissolved protein content in the leaves of two ecotypes presented the same changing trend as the Cd treatment concentration,both touched tile bottom at the 20 mg/kg treatment. With the increase of the Cd treatment concentration, the dissolved sugar content in non-mining ecotype decreased before increased,whose concentration was all significantly lower than control in 10 -80 mg/kg treatment （ P 〈 0.05 ）. While the content of dissolved sugar content increased before decreased with the increase of Cd treatment concentration, the dissolved sugar content in two eeotypes of Miscanthus floridulus responsed differently to Cd treatment concentration. [ Conclusion ] The heavy metal of Cd in the mining ecotype of Miscanthus floridulus was better than that of the non-mining eeotype.

关 键 词：CD 五节芒 生理响应 生态型 

分 类 号：S158.4[农业科学—土壤学]