一种烟草漂浮苗病害的鉴定  

Identification of Floating Seedling Disease in Tobacco

作　　者：夏振远[1] 莫笑晗[1] 雷丽萍[1] 刘勇[1] 祝明亮[1] 

机构地区：[1]云南省烟草农业科学研究院,云南玉溪653100

出　　处：《安徽农业科学》2010年第31期17520-17521,17544,共3页Journal of Anhui Agricultural Sciences

基　　金：云南省烟草公司项目(07A08)

摘　　要：[目的]对一种在烟草漂浮苗上发现的病害的病原进行分离和鉴定,为该病的防治提供依据。[方法]将分离得到的菌株按照柯赫氏法则进行致病性测定,通过B iolog测定、16SrDNA序列分析和其他生理生化方法对病原菌进行鉴定。[结果]经柯赫氏法则试验证明分离得到的3-3菌株是引起该漂浮苗病害的病原菌,经B iolog鉴定、生理生化分析符合胡萝卜果胶杆菌胡萝卜亚种的特征,16SrDNA序列分析表明3-3与胡萝卜果胶杆菌胡萝卜亚种的菌株Kun28213相似程度最高,达99.9%。[结论]综合几种方法的鉴定结果,引起烟草漂浮苗病害的病原为胡萝卜果胶杆菌胡萝卜亚种,该病害是在国内首次报道。根据英文的名称,将该病害命名为烟草黑脚病。[ Objeefive ] The pathogen of disease found in the floating seedlings of tobacco was isolated and identified to provide the basis for the control of the disease. [ Method ] The pathogenicity of the isolated strain was determined according to Kochg rules,and the pathogen was i- dentified through Biolog determination, 16SrDNA sequence analysis and the other physiological and biochemical methods, [ Result ] Through Koch＇s test,the isolated 3-3 strain was verified to be the pathogen causing floating seedling disease,which was consistent with the characteris- tics of Pectobacterium carotovorum subsp. Carotovorum fllrough Biolog determination and the other physiological and biochemical methods. 16SrDNA sequence analysis showed that 3-3 strain had the highest similarity with P. carotovorum subsp, carotovorum strains Kun28213 （accession number GU936996） ,reaching 99.9%. [ Conclusion ] Base on the identification results of several methods, the pathogen causing floating seedling diseases was P. carotovorum subsp. Carotovorum,the disease was first reported in China. According to the English name of the disease, the disease was called as tobacco Blackleg disease.

关 键 词：烟草 漂浮苗 黑脚病 鉴定 

分 类 号：S604.3[农业科学—园艺学]