大孔树脂法分离纯化海燕总皂苷的研究  

Purification of Asterosaponin from Asterina pectinifera by Macroporous Resin

作　　者：李敏晶[1,2] 韩艳玲[1] 谭成玉[1,2] 刘远[1,2] 

机构地区：[1]大连海洋大学海洋环境工程学院,辽宁大连116023 [2]大连海洋大学辽宁省高校近岸海洋环境科学与技术重点实验室,辽宁大连116023

出　　处：《安徽农业科学》2010年第31期17736-17738,共3页Journal of Anhui Agricultural Sciences

基　　金：辽宁省教育厅科学技术研究项目(2009A177)

摘　　要：[目的]探讨大孔树脂法分离纯化海燕总皂苷的最佳工艺。[方法]采用优选树脂分离纯化海燕总皂苷，以分光光度法测定海燕总皂苷的含量。[结果]D-101型大孔树脂吸附纯化海燕总皂苷的效果最好，其工艺参数为：饱和吸附量为65．8mg／g，解吸率N97．66％，洗脱时上样原液pH值为6～7，洗脱液为体积分数为70％的乙醇，洗脱液用量为6BV；洗脱液流速为6BV／h。[结论]所建立的分离纯化工艺简单可行，为海燕总皂苷的工业生产提供了依据。[ Objective ] The researeh aimed to study the optimum technology conditions for purification of asterosaponin from Asterina pectinifera using macroporous resin. [ Method ] By analysis the contents of asterosaponin from Asterina pectinifera by UV spectrophotometry, the purification by macroporous resin was studied. [ Results] The optimum purification conditions were as fullows： macroporous resin of D-101, maxinmm adsorption amount of 68.5 rag/g, desorption rate of 97.66% , pH of stock solution of 6 -7, eluent of 75% ethanol, eluent volume of 6 BV, and flow rate of 6 BV/h. [ Conclusion I The optilnized process established in this study was simple and feasible ,which provides the basis for the industrial production of asterosaponin from Asterina pectinifera.

关 键 词：大孔树脂 海燕 总皂苷 纯化 

分 类 号：Q959.266[生物学—动物学]