内皮素鞘内注射造成大鼠截瘫  

Hindlimb Paralytic Effect of Endothelin following Spinal Subaraehnoid Injection in the Rat

作　　者：盛树力[1] 王霄虹[1] 陈雁江[1] 周力[1] 伍洁[2] 苏敏成 

机构地区：[1]北京市神经外科研究所 [2]北京儿童医院

出　　处：《首都医学院学报》1990年第4期247-250,共4页

摘　　要：我们以往的研究表明,内皮素(ET)的缩血管效应、对心脏的毒性作用和ET脑室注射引起的血压降低,脑血流量减少的作用均可被CGRP对抗。本实验观察了鞘内注射(I.T.)ET对神经功能的影响和对血压、心率、血中AVP、ANP水平的影响,以及CGRP是否能改善ET引起的双下肢功能障碍。给大鼠脊髓蛛网膜下腔注射ETl0μl,5min内可以引起大鼠后肢瘫痪,其程度与ET剂量有关。30ng以下大鼠一般在30～60min内恢复,而40ng以上造成的后肢瘫痪几乎是不可逆的。I.T.注射30ng ET引起的后肢瘫痪,可以被I.T.注射CGRP400ng/10μl明显改善。病理切片显示,I.T.注射ET40ng 4h后,大鼠脊髓具有缺血表现,提示ET造成的后肢瘫痪可能是ET强烈收缩血管所致。我们又观察到:I.T注射ET30ng/10μl血压有不同程度的提高、心率加快,而i.v.注射ET同等剂量对血压无影响。说明I.T.注射ET30ng/10μl引起的这些改变可能是ET所致的神经效应。Our previous studies have demonstrated that CGRP could attenuate the effects of ET on blood vessels, regional cerebral blood flow and heart. This contribution of CGRP resulted from a most potent dilation of blood vessels. In this research, rats were administrated ET 10μl into the spinal subarachnoid space at lumbar-sacral region. Some rats were monitored BP, heart rate and the contents of plasma ANP and AVP were determined. The rat appeared variably paraplegic a few minutes after giving ET. When the ET dose was over 40ng, they had a high mortality. When no more than 30ng was administrated, the mortality of rat was 20%. Among survivors, 98% had reversible paraplegia, 25min after CGRP administration, a significantd egree of recovery of paraplegia recovered. Pathological examination 4h after onset of paraplegia showed serious ischemia of spinal cord. It indicates the hindlimb paraplytic effect of ET is probably due to potent constriction of blood vessels. We also observed a few increase of BP and heart rate in the rats I. T. injected ET, but no change after i. v. administration of the same dose of ET. It suggests that the pressor to I. T. ET did not resulted from I. T. ET to peripheral blood flow, it is possible to be induced by the neuronal reflex of I. T. injection of ET.

关 键 词：内皮素 鞘内注射 瘫痪 

分 类 号：R972.9[医药卫生—药品]