高通量悬浮芯片技术检测多农兽药残留  

High-throughput Suspension Array Technology for Simultaneous Detection of Multiple Pesticide and Veterinary Drug Residues

作　　者：刘楠[1] 苏璞[1] 朱茂祥[2] 杨陟华[2] 潘秀颉[2] 王红勇[1] 晁福寰[1] 高志贤[1] 

机构地区：[1]天津市环境与食品安全风险监控技术重点实验室,军事医学科学院卫生学环境医学研究所,天津300050 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《分析化学》2010年第5期673-677,共5页Chinese Journal of Analytical Chemistry

基　　金：国家科技支撑计划(No.2006BAK02A09);国家自然科学基金(Nos.30771810,30800915)资助项目

摘　　要：建立了一种同时可检测多农兽药残留的高通量悬浮芯片技术。以常用的7种农兽药为待测靶标物,通过优化条件,使抗原抗体基本完全反应,操作简便快捷,最多可同时检出7种农兽药残留靶标物;实验结果表明,高通量悬浮芯片检测标准回归曲线方程和相应的决定系数R2表现良好;在对含有多种农兽药残留的不同盲样的检测中,检测浓度值与实际浓度的相对偏差较小。高通量悬浮芯片技术为多农兽药残留的快速检测提供了新方法,此系统可用于多农兽药残留实际样品的初步检测,整个检测过程仅耗时1～2h,基本上满足了多农兽药残留检测的灵敏、特异、快速和高效的需求,具有广阔的应用和发展前景。A novel high-throughput suspension array technology has been established for simultaneous detection of multiple pesticide and veterinary drug residues. Seven kinds of pesticide and veterinary drugs which were commonly used were regarded as targets. Antigen-antibody reactions were nearly completed by optimization of the reaction conditions. Up to seven kinds of residues could be rapidly and simultaneously determined with a simple and rapid operation. It showed that, standard regression curves of the high throughput suspension array and the coefficients of determination-R2 were performed well. In the tests of different samples with multiple pesticide and veterinary drug residues, the relative errors of the found and the real values were relatively small. The high throughput suspension array provides a novel method for rapid detection of pesticide and veterinary drugs residues within 1-2 h. The requirement for sensitive, spcific, rapid and effident for residues are basically satisified with wide application and development.

关 键 词：悬浮芯片 高通量 微球 残留 中位荧光强度值 

分 类 号：S859.84[农业科学—临床兽医学]