阿特拉津分子印迹固相萃取柱的制备及应用  

Preparation and Application of Atrazine Molecularly Imprinted Solid Phase Extraction Pillar

作　　者：王颜红[1] 霍佳平[1,2] 张红[1] 王世成[1] 司士辉[2] 林桂凤[1] 李国琛[1] 刘伟[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,沈阳110016 [2]中南大学化学化工学院,长沙410083

出　　处：《分析化学》2010年第5期678-682,共5页Chinese Journal of Analytical Chemistry

基　　金：中科院重要方向项目(No.KSCX2-YW-N-035);财政部专项绿色农业科学研究与示范项目(No.LSNY-2007-10);沈阳市大型仪器专项(No.2008GX-034)的资助

摘　　要：以阿特拉津为模板分子,α-甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,偶氮二异丁腈为引发剂,65℃聚合17h,制备了对阿特拉津具有特异性识别的分子印迹聚合物。将聚合物研磨、过筛、洗涤并装柱,制备分子印迹固相萃取柱,其最大结合量比游离态的聚合物微球高600倍,结合时间为游离态的1/60,采用液相色谱法检测其回收率达90%;用于富集水样中含量低于2×10-8mol/L的待测物,其回收率也可达到89.3%。与市售C18柱相比,分子印迹固相萃取柱回收时间仅为C18柱的1/3,试剂用量为其1/4,但回收率更好,净化更彻底,减少了杂质峰对分析的影响,提高了灵敏度,利于仪器的保护和保养。应用此方法对实际农业水样进行了检测,结果满意。The molecular imprinted polymers（MIPs） was synthesized by polymerization for 17 h using atrazine as template, α-methacrylic aid（MAA） as monomer, ethylene glycol dimethacrylate（EDMA） as cross-linker and 2,2-azobisisobutyronitrile（AIBN） as initiator. This polymer could specially recognize atrazine. The polymers was grinded, sieved, washed and packed into cartridges. The adsorbability of the solid phase extraction（SPE） pillar being stuffed with the polymers was 600 times compared with that of the polymer sphere, and the extraction time of this SPE pillar was only 1/60 compared with that of polymer sphere, the recovery was 90% testing by HPLC. The SPE pillar could enrich atrazine at the concentration less than 2×10-8 mol/L in the water sample with the recovery of 89.3%. In comparison with C18 pillar, the MIPs-SPE pillar used only 1/3 time and 1/4 reagent, and got higher recovery, as well, purified the sample water more drastically, decreased the disturbance of peculiar peaks, improved the sensitivity and could protect the instrument against damage. And the water for agricultures could be tested using this method.

关 键 词：分子印迹聚合物 阿特拉津 模板分子 固相萃取 液相色谱 

分 类 号：X839.2[环境科学与工程—环境工程]