高效液相色谱法检测水体中微囊藻毒素  

Determination of Microcystins in Aquatic Environment with High Performance Liquid Chromatography

作　　者：梁丽丽[1] 弓爱君[1] 李红梅[2] 曹艳秋[1] 李宝芹[1] 

机构地区：[1]北京科技大学,北京100083 [2]中国计量科学研究院,北京100013

出　　处：《分析化学》2010年第5期740-742,共3页Chinese Journal of Analytical Chemistry

基　　金：国家标准物质资源共享平台建设项目(No.2005DKA21501);北京市教委产学研项目资助

摘　　要：建立了高效液相色谱检测水体中微囊藻毒素(Microcystins,MCs)的方法。选择XAD-2树脂为富集树脂,采用四氯化碳-10%乙醇-10%丙酮连续淋洗,获得较好的杂质去除效果,采用90%甲醇可以将树脂柱上的MCs完全洗脱。HPLC采用V(0.1%TFA):V(甲醇)=42∶58混合溶液为流动相进行等度洗脱,MC-RR和MC-LR取得较好分离效果,回收率均达到90%以上。水样富集2000倍后,方法的检出限为0.05μg/L。本方法操作简便、灵敏度高、检测速度快。The determination method for microcystins （MCs） in aquatic environment with high-performance liquid chromatography was improved. The results showed that good effects on the rinse of impurity was obtained by using carbon tetrachloride-10% ethanol-10% acetone as the eluate, and all the MCs in XAD-2 resin column could be eluted by 90% methanol-water solution. In the experiment, MC-RR and MC-LR could be totally separated at isocratic elution with mobile phase V（0.1% trifluoroacetic acid）∶V（methanol）=42∶58 and the recoveries both reached 90%. The detection limits for both of the MCs were 0.1 μg/L after the water samples were condensed 6000 times. The detection method is simple, sensitive and fast.

关 键 词：微囊藻毒素 高效液相色谱 

分 类 号：X832[环境科学与工程—环境工程]