低温处理促使五味子愈伤组织再分化  

Low Temperature Treatment on Callus Redifferentiation of Schisandra chinensis

作　　者：李国泰[1] 顾地周[1] 

机构地区：[1]通化师范学院,通化134002

出　　处：《东北林业大学学报》2010年第5期60-61,共2页Journal of Northeast Forestry University

基　　金：吉林省科技厅资助项目(20040904-21-2)

摘　　要：以五味子的嫩茎段基部诱导产生的愈伤组织为试材,接种于1/4MS的培养基中进行低温处理,并应用均匀设计筛选出最适宜的处理温度和时间,再将处理后的愈伤组织转接到培养基MS+6-BA1.50mg·L-1+IBA0.02mg·L-1中进行愈伤组织再分化培养。结果表明:愈伤组织经-4℃的低温处理53d后,再经50d的培养分化出大量的再生芽苗。Callus from tender stem base of Schisandra chinensis （Turcz.） Baill was inoculated into 1/4MS at low temperature,to screen the most suitable treatment temperature and time by uniform design. Then the callus was transferred to MS medium supplemented with 1.50mg·L-1 6-BA and 0.02mg·L-1 IBA for shoot differentiation. Results showed that,after 50 days of cultivation,a large number of shoots were regenerated from calluses treated at minus 4 degrees C for 53 days.

关 键 词：五味子 愈伤组织 再分化 均匀设计 

分 类 号：Q254[生物学—细胞生物学] Q943.1