青海不同地区秦艽、麻花艽中落干酸和龙胆苦苷的含量测定  

Compare with the Active Constituents in Different Districts of Gentiana Macrophylla Pall

作　　者：俞青芬[1] 

机构地区：[1]青海民族大学化学系,青海西宁810007

出　　处：《江西师范大学学报（自然科学版）》2010年第2期174-177,共4页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：国家自然科学基金(30760195)资助项目

摘　　要：采用Kromasil-C18（250 mm×4.6 mm,5μm）色谱柱,流动相为甲醇-0.04%磷酸水溶液系统,流动相梯度洗脱程序为在15 min内甲醇从体积23%线性变化为30%,检测波长为238 nm,流速为1 mL/min,柱温为35℃,建立了对青海不同地区秦艽、麻花艽中落干酸和龙胆苦苷的高效液相色谱测定方法.结果表明：落干酸和龙胆苦苷的回收率分别为95.19%和96.01%;RSD分别为0.57%和0.33%;线性范围分别为0.198 0~5.544 0μg（r=0.999 9）和0.026 0~0.733 6μg（r=0.999 9）.The sample were separated on the column of Kromasil-C18（250 mm×4.6 nm,5 μm） which eluted with elutde with methanol and water（0.04% phosphoric acid）.The ratio of methanlo and water increased from 23% to 30% in 15 min with detected wavelength at 238 nm,flow rate at 1 mL/min,column temperature at 35 ℃,to establish a quantitative method of simultaneously determination of loganic acid and gentiopicroside in different districts of Gentiana Macrophylla Pall by RP-HPLC.The average recoreries were 95.19%,96.01%（RSD=0.57% and 0.33%） of the loganic acid and the gentiopicroside,two compounds were baseisolated.The linear rangs of loganic acidand gentiopicrolatde.The linear rangs of loganic acid and gentiopicroside were 0.198 0-5.544 0 μg（r=0.999 9）,0.026 0-0.733 0 μg（r=0.999 9） respectively.

关 键 词：HPLC 秦艽 落干酸 龙胆苦苷 

分 类 号：O655[理学—分析化学]