氮素浓度对黄檗幼苗生长及氮代谢相关酶类的影响  

Effects of Nitrogen Concentrations on Nitrogen Metabolism-related Enzymes and Growth of Phellodendron amurense Seedlings

作　　者：李霞[1] 阎秀峰[2] 

机构地区：[1]吉林师范大学,四平136000 [2]东北林业大学

出　　处：《东北林业大学学报》2010年第6期40-42,共3页Journal of Northeast Forestry University

基　　金：国家自然科学基金项目(30271045);黑龙江省杰出青年基金项目(JC-02-11)资助

摘　　要：在温室采用水培法以5种氮素营养水平(1、4、8、16和32mmol/L)培养黄檗(Phellodendron amurense)幼苗,研究了不同氮素形态对黄檗幼苗生长及氮代谢相关酶类的影响。结果表明:经氮素浓度为16mmol/L处理的株高、茎直径和全株生物量均达到最大值;氮素浓度为1mmol/L时,黄檗幼苗的生长状况最差。随氮素浓度的升高,叶片中铵态氮、硝态氮和可溶性蛋白质量分数也升高,但当升高到32mmol/L,可溶性蛋白质量分数不会继续升高。总体来看,氮素浓度升高,可在一定程度上提高硝酸还原酶(NR)和谷氨酰胺合成酶(GS)的活性。An experiment was conducted to study the effects of nitrogen forms on nitrogen metabolism-related enzymes and growth of Phellodendron amurense seedlings cultivated at five different nitrogen concentrations （ 1,4,8,16 and 32 mmol/L） in a greenhouse by hydroponic method. Result showed that the height,caudex diameter,and total biomass of P. amurense seedlings reached maximum values at a nitrogen concentration of 16 mmol/L,while P. amurense seedlings grew worst at 1 mmol/L. The contents of NH4^＋—N,NO3^-—N and soluble protein increased with the increase of nitrogen concentration,however,the content of soluble protein would not increase when the nitrogen concentration up to 32 mmol/L. As a whole,the increase of nitrogen concentration could enhance the activities of nitrate reductase and glutamine synthetase to a certain extent.

关 键 词：黄檗(Phellodendron amurense) 氮素浓度 氮素代谢 

分 类 号：S567.19[农业科学—中草药栽培]