发酵支原体的套式PCR检测研究及其临床应用  

Detection of Mycoplasma fermentans by nested polymevase chain reaction.

作　　者：秦玲[1,2] 赵季文[1,2] 糜祖煌[1,2] 

机构地区：[1]无锡市克隆遗传技术研究所 [2]南京铁道医学院流行病学教研室

出　　处：《中国优生与遗传杂志》1999年第2期28-29,共2页Chinese Journal of Birth Health & Heredity

摘　　要：发酵支原体（Ｍｆ）已从孕妇的胎盘和羊水中分离检测到，这一发现揭示Ｍｆ存在宫内感染的可能性。Ｍｆ的人工培养更难于其它致病性支原体。为此，我们以Ｍｆ的１６ＳｒＲＮＡ基因为靶基因建立了Ｍｆ－ＤＮＡ的套式检测技术。结果表明Ｍｆ－ＤＮＡ的ＰＣＲ法检测是一种灵敏、特异、简便和有价值的手段。Mycoplasma fevmentans(Mf) has been detected in the placentas and amniotic fluid, a finding that suggests the potential for transmission in utero .Mf is apparently move difficult than other pathogenic mycoplasmas to cultivate on antificial media.We established an nested polymerase chain reaction method of Mf-16SrRNA-gene detection. The result is a sensitive,specific,simple and valuable tool.

关 键 词：发酵支源体 套式PCR 宫内感染 

分 类 号：R714.251[医药卫生—妇产科学] R375[医药卫生—临床医学]