银掺杂聚L-酪氨酸修饰电极的制备及对尿酸的测定  

Preparation of Silver Doped Poly(L-tyrosine) Modified Electrode and Its Application for the Determination of Uric Acid

作　　者：田相星[1] 马伟[1] 孙登明[1] 

机构地区：[1]淮北师范大学化学与材料科学学院,安徽淮北235000

出　　处：《分析科学学报》2010年第6期653-656,共4页Journal of Analytical Science

基　　金：安徽省科技计划重点项目(No.07020203011);安徽省高校省级自然科学重点项目(No.KJ2008A122)和一般项目(No.KJ2008B87ZC)

摘　　要：利用循环伏安法将银和L-酪氨酸聚合修饰在玻碳电极表面,制成银掺杂聚L-酪氨酸(Ag-PLT/GCE)修饰电极,研究了尿酸在该电极上的电化学行为,建立了循环伏安法测定尿酸的新方法。在pH=3.0的磷酸盐缓冲溶液中,扫描速率为100mV/s时,尿酸在该修饰电极上产生一氧化峰,Epa=0.637V(vs.Ag/AgCl)。用循环伏安法进行测定时,峰电流与尿酸浓度在8.0×10-7～1.0×10-5mol/L和1.0×10-5～1.0×10-4mol/L范围内呈良好的线性关系,检出限为3.0×10-7mol/L。方法用于尿样中尿酸的测定,结果满意。The silver doped poly（L-tyrosine）modified electrode（Ag-PLT/GCE）was prepared by cyclic voltammetric method.The voltammetric behaviors of uric acid were studied at the silver doped poly（L-tyrosine） modified electrode.In phosphate buffer solution at pH 3.0,the oxidation peak potential was Epa=0.637V（vs. Ag/AgCl）at the scan rate of 100mV/s.The linear ranges for the determination of uric acid were 8.0×10^-7～ 1.0×10^-5 mol/L and 1.0×10^-5～1.0×10^-4 mol/L.The detection limit was 3.0×10^-7 mol/L.The method was applied to the determination of uric acid in urine with satisfactory results.

关 键 词：尿酸 银掺杂 L-酪氨酸 聚合物修饰电极 循环伏安法 

分 类 号：O657.15[理学—分析化学]