抗氧化水平对小麦幼苗耐缺铁及铁累积能力的影响  

Effects of antioxidative levels on iron deficiency tolerance and iron accumulation in wheat (Triticum aestivum L.) seedlings

作　　者：于洋[1] 张玉秀[1] 殷恒霞[2] 李霞[1] 

机构地区：[1]中国矿业大学(北京)化学与环境工程学院,北京100083 [2]青海师范大学生命与地理科学学院,西宁810008

出　　处：《中国生态农业学报》2010年第6期1256-1260,共5页Chinese Journal of Eco-Agriculture

基　　金：国家科技重大专项转基因专项(2009ZX08009-130B)资助

摘　　要：以小麦品种"石4185"和"沧6001"为试验材料,采用水培方法,分析了抗氧化水平对小麦幼苗耐缺Fe和Fe累积能力的影响。结果表明:Fe缺乏引起小麦根部活性氧(ROS)累积,导致根部产生过氧化伤害,同时降低叶片中叶绿素含量。与"石4185"相比,"沧6001"具有相对较低的ROS水平和较高的叶绿素含量。缺Fe条件下,"沧6001"比"石4185"诱导出更多的根毛和侧根,并具有较高Fe累积。抗氧化酶活性分析结果表明,缺Fe条件下,"沧6001"比"石4185"具有更高的SOD、CAT和APX活性。在缺Fe条件下添加抗氧化剂NAC或AsA提高了幼苗中的Fe含量,表明抗氧化水平的提高促进了小麦幼苗对Fe的吸收和累积。本研究对于理解小麦对缺Fe胁迫响应的生理机制及筛选耐Fe缺乏和高Fe累积的小麦品种具有指导意义。In this study, we choose wheat cultivars ＂Shi 4185＂ and ＂Cang 6001＂ as materials to examine effects of antioxidative levels on iron deficiency tolerance and iron accumulation in wheat （Triticum aestivum L.） seedlings. The results show that iron deficiency markedly increases reactive oxygen species （ROS） level in roots, thereby induces oxidative damage in roots of wheat seed-lings and reduces the chlorophyll content in leaves. Compared to ＂Shi 4185＂, ＂Cang 6001＂ seedlings have lower ROS level, higher chlorophyll content, more lateral roots and higher leaves iron accumulation, as well as higher SOD, CAT and APX activities as exposed to iron deficiency. Supplementation of antioxidants such as ascorbic acid （AsA） and N-acetyl-L-cysteine （NAC） increases Fe content in wheat seedlings, suggesting higher antioxidative level improves Fe absorption and accumulation in wheat seedlings. This study has positive significance for further understanding the physiological mechanisms of wheat in response to iron deficiency stress and screening wheat cultivars for high iron deficiency tolerance and iron accumulation.

关 键 词：小麦 铁缺乏 抗氧化酶 铁累积 叶绿素 活性氧 

分 类 号：Q945.78[生物学—植物学]