双抗夹心ELISA法测定水资源、食品及饲料中总甾体激素含量  

ELISA Detection of Steroidal Hormones in Water Resources,Food and Feed

作　　者：马德章[1] 刁昱文[2] 杨佳新[1] 王宝德 于源华[1] 

机构地区：[1]长春理工大学生命科学技术学院,长春130022 [2]吉林大学畜牧兽医学院 [3]中国石油管道长吉分公司

出　　处：《应用化学》2010年第12期1466-1469,共4页Chinese Journal of Applied Chemistry

基　　金：吉林省科技厅重大项目(20096012)资助

摘　　要：基于睾丸酮丛毛单胞菌(C.test)具有降解甾体类物质的特性,其细胞中3α-羟基类固醇脱氢酶/碳酰基还原酶(3α-HSD)是诱导酶,建立了以3α-HSD的测定总甾体激素含量的双抗夹心酶联免疫反应(ELISA)方法。实验结果表明,睾丸酮类甾体激素的量在0.5～600μg/g之间,与3α-HSD酶表达量具有明显线性关系。应用这种方法对多种水资源、饲料、肉食品进行了检测。检测结果表明,松花湖水中的睾丸酮类甾体激素含量为34.5μg/g,明显高于其它水系;被测饲料中睾丸酮类甾体激素含量均大于2μg/g;肉类样品的检测结果均低于检测下限。本方法与高效液相色谱法的测定结果相符。Comamonas testosteroni secretes 3α-HSD/CR when induced by steroid hormones,then it has the characteristics to degrade steroid hormones.This work developed a Enzyme-linked immunosorbent assay（ELISA） method to detect the steroidal hormones in water,food and animal feed.The results show that the content of testosterone and 3α-HSD enzyme expression have a linear relationship.The linear range is within 0.5～600 μg/L.Adopting this method,the study detect the steroidal hormones in water,food and feed.Content of testosterone in Songhua Lake is 34.5 μg/L,which is higher than others.Content of testosterone in the 8 animal feed is more than 2 μg/L.Meat sample test shows that the amount of steroidal hormones is below the detection limit.This result from this method is consistent with that from HPLC.

关 键 词：甾体激素 酶联免疫反应 3α-羟基类固醇脱氢酶/碳酰基还原酶 

分 类 号：O652[理学—分析化学]