崇明水仙(Narcissus tazetta var.chinensis)盆栽褐枯病病原的鉴定  

Identification of wilt pathogens of potted Chongming narcissus(Narcissus tazetta var.chinensis)

作　　者：马腾飞[1] 许振[1] 冯琳燕[1] 李小方[1] 孙越[1] 许玲[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《华东师范大学学报（自然科学版）》2010年第6期64-71,82,共9页Journal of East China Normal University(Natural Science)

基　　金：上海市科技兴农重点攻关项目(沪农科攻字2005第1-2号)

摘　　要：依据柯赫氏法则对崇明水仙盆栽中叶褐枯病病原菌进行分离,得到3个可造成鳞茎和叶不同程度腐烂的致病真菌菌株.应用形态学鉴定法和真菌rDNA-ITS序列分析及ISSR分子标记技术进行鉴定,结果1株为丛赤壳属的丛赤壳菌(Nectria gliocladioides),2株为镰刀菌属的串珠镰刀菌胶胞变种(Fusarium moniliforme var.subglutinans).其中Nectria gliocladioides为引起崇明水仙盆栽枯萎病的主要病原真菌,该菌的最适生长温度为25℃,低于5℃和高于40℃菌丝均不能生长.研究结果证实ISSR技术可用于区分菌株间的种属差异,可作为病原真菌鉴定的分子辅助手段.Using morphologic characters and internal transcribed spacers（ITS） sequence analysis and Inter-simple-sequence-repeat（ISSR）markers,3 isolates wilt pathogens in potted plant of Chongming narcissus were detected.One was Nectria gliocladioides,while other two were Fusarium moniliforme var.subglutinans.Pathogenic test resulted in that N.gliocladioides was the advantage pathogen,and its optimum growth temperature is 25℃.The hypha couldn＇t growth while the temperature is lower than 5℃or higher than 40℃.The study would be helpful to prevent and cure blight of Chongming narcissus potted plant.ISSR markers are useful for distinguishing the pathogen stains.It could be a molecular assistant means for identification of fungal and species.

关 键 词：水仙 褐枯病 ISSR RDNA-ITS 

分 类 号：S432.43[农业科学—植物病理学]