生长素在芦笋组培扩繁体系中的影响  

Effects of auxins on the propagation of Asparagus officinalis L.

作　　者：王婧宇[1] 张雪平[1] 杨如[1] 李小方[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《华东师范大学学报（自然科学版）》2010年第6期101-108,共8页Journal of East China Normal University(Natural Science)

基　　金：上海市科委专项重点项目(07DZ12043)

摘　　要：对芦笋UC308侧芽增殖和生根诱导中生长素的作用规律进行研究.发现不添加任何外源生长素,芦笋仍有较高的侧芽萌发率;而对于愈伤组织的形成,外源生长素是必不可少的.使用0.05 mg/LNAA进行侧芽诱导,浓度为1.0 mg/LNAA进行愈伤组织的诱导较为适宜.在侧芽诱导过程中,NAA浓度大于2.0 mg/L时,不会对后期的不定根的形成产生影响.比较4种生长素IAA,NAA,IBA和2,4-D对芦笋生根诱导效果,发现IBA最为适宜,2,4-D对芦笋生根无明显促进作用.The roles of auxin played in the propagation of asparagus were studied.Exogenous auxin was necessary for the formation of calli,while it wasn＇t necessary during proliferation of shoots.The best induction of shoots was achieved with 0.05 mg/L NAA,and more calli induction needed over 1.0 mg/L NAA.The concentration of NAA in the regeneration of shoots hadn＇ t further influence on rooting.Comparing the four sorts of auxins,NAA,IAA,IBA and 2,4-D during roots induction of asparagus,IBA is the best choice both in the quality and rate of rooting.

关 键 词：芦笋 生长素 组织培养 生根 

分 类 号：Q94[生物学—植物学]