HPLC法测定近视康合剂中柴胡皂苷d的含量  

Using HPLC to Determination Saikosaponin d Content in Jinshikang Mixture

作　　者：田静[1] 王晓梅[1] 胡勤[1] 

机构地区：[1]湖南省儿童医院,湖南长沙410007

出　　处：《中医药导报》2010年第11期82-83,共2页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:建立近视康合剂的质量控制方法。方法:样品经甲醇超声提取,使用C18色谱柱,乙腈-水(40∶60)为流动相,检测波长208 nm,流速1 mL·min-1,柱温30℃。结果:柴胡皂苷d在(0.496 g-2.48 g)范围内呈良好的线性关系,r=0.9998(n=5);平均加样回收率为99.9%。结论:样品处理方法合理,方法学考察符合定量要求,结果准确,可靠、简便、易行,可作为近视康合剂质量控制标准。Objective：To establish a quality control method of Jinshikang mixture.Methods：The sample was extracted with methanol under ultrasonic management.Chromatographic conditions： C18 column,acetonitrile-water（40∶60） as the mobile phase,detector at 208 nm,flow rate at 1 mLomin^-1,temperature of column at 30 ℃.Results The peak area of Saikosaponind show a good linearity in the range of（0.496 g-2.48 g）,r=0.9998（n=5）;recovery rate is 99.9%.Conclusion：Sample preparation method is reasonable,methodological study meet the quantitative requirements,accurate,reliable,and simple,the method provides a quality control standard for Jinshikang mixture.

关 键 词：近视康合剂 柴胡皂苷D HPLC 

分 类 号：R284.1[医药卫生—中药学]