ISSR和RAPD分子标记技术在不同君子兰品种遗传多样性上的应用  

Application on the Genetic Diversity of Different Species of Clivia miniata by ISSR and RAPD Molecular Markers

作　　者：郑玉红[1] 钱美华[1] 李莹[1] 顾永华[1] 彭峰[1] 

机构地区：[1]江苏省中科院植物研究所南京中山植物园,南京江苏210014

出　　处：《北方园艺》2010年第23期136-139,共4页Northern Horticulture

基　　金：江苏省农业科技成果转化资助项目(CC20092020)

摘　　要：采用ISSR标记和RAPD标记技术研究了15个君子兰品种的遗传多样性。结果表明：从55条寡聚核苷酸引物中筛选出10条简单重复序列引物，共扩增出65条带，平均每条引物扩增出6．5条带，其中5．4条多态性带，多态性比率为81．95％；从55条寡聚核苷酸引物中筛选出11条多态性引物，共扩增出47条带，平均每条引物扩增出4．27条带，其中32条多态性带，多态性比率为67．88％。POPGEN软件计算出君子兰品种间遗传距离为0．1123-0．6330，平均值为0．3263。基于遗传相似系数的UPGMA聚类分析将15个品种明显聚为二组，国兰系列品种为一组，日本兰系列品种组成另一组。Genetic and variance on 15 cultivars of Clivia miniatawere studied by ISSR and RAPD marker. The results showed that the 65 loci were identified with 10 simple sequence repeat primers screened from 55 primers,out of which 54 were polymorphic and the percentage of polymorphic sites （PPS） was 81. 95%;with each primers 6. 5 bands were averagely amplified. 47 loci were identified with 11 oligodeoxynucleotides primers screened from 55 primers,out of which 32 were polymorphic and the PPS was 67. 88%; with each primers 4. 27 bands were averagely amplified. The Nei＇s genetic distance among the 15 cuhivars calculated by POPGEN was 0. 1123-0. 6330 with an average of 0. 3263. Cluster analysis indicated that the 15 cultivars could be divided into 2 groups. The results provided molecular data for the breeding of new cultivars of C. miniata.

关 键 词：君子兰 品种 ISSR RAPD 遗传变异 

分 类 号：S682.13[农业科学—观赏园艺]