利用RT-PCR对脱毒草莓苗病毒检测研究  

Study of Detection Virus on Seedling of Virus-free Strawberry by RT-PCR

作　　者：陈晓军 王敬东 马洪爱 陈虞超 宋玉霞 

机构地区：[1]宁夏自治区农业生物技术重点实验室,宁夏银川750002

出　　处：《北方园艺》2010年第23期146-148,共3页Northern Horticulture

基　　金：宁夏自然科学基金资助项目(NZ0974);宁夏科技攻关资助项目(KGZ-16-07-02)

摘　　要：利用RT-PCR技术,以草莓肌动蛋白基因序列为内标,结合4种草莓病毒特异性扩增,检测草莓组培脱毒苗是否脱毒情况。结果表明:此内标与这4种草莓病毒的特异性扩增的结合,能较好达到病毒检测的目的,减少了结果判断的盲目性,为准确、快速检测草莓脱毒苗提供了一套较为完整的试验方法。Using RT-PCR technology to the actin gene sequence of strawberry as the internal standard, with 4 strawberry virus-specific amplification, the virus-free conditions of virus-free eedlings of strawberry were detected. The results showed that the internal standard and the specific amplification of strawberry virus binding, can better achieve the purpose of virus detection, reducing the blindness of estimate on results, for accurate, rapid detection of virus-free seedlings of strawberry to provide a set more complete test method.

关 键 词：草莓脱毒苗 RT-PCR SMoV SMYEV SVBV SCV 

分 类 号：S668.4[农业科学—果树学]