高效液相色谱法测定血浆中同型半胱氨酸  

Determination of Homocysteine in Plasma by High Performance Liquid Chromatography

作　　者：潘峰[1] 孙玮[1] 张青[1] 罗婧[1] 王丽婷[1] 王芳[1] 胡茂华[1] 张正治[1] 

机构地区：[1]第三军医大学基础部中心实验室,重庆400038

出　　处：《氨基酸和生物资源》2010年第4期55-57,共3页Amino Acids & Biotic Resources

摘　　要：建立了一种高效液相色谱-库仑阵列电化学测定血浆中同型半胱氨酸的方法。采用3-2-羧乙基膦作为还原剂还原巯基间形成的二硫键,用0.1 mol.L-1HC lO4来沉淀血浆蛋白,离心取上清,进样分析。在0.40~50.00μmol.L-1范围内,线性关系良好。加标平均回收率是98.43%,RSD%是4.35%。该方法简单、快速、灵敏。A high performance liquid chromatography（HPLC） with coularry electrochemical detection method for quantitative determination of human plasma homcysteine（Hcy） has been developed.Hcy was measured after the chemical reduction of all disulfide bonds with 100 μg· mL-1 tris（2-carboxyethyl）-phosphine hydrochloride（TCEP） and protein precipitation with 0.1 mol·L-1 HClO4.There was a good linear relationship between the content of Hcy and its peak area（r=0.999 8） in the range of 0.40-50.00μmol·L-1.The average recovery rate was 98.43% and the relative standard deviation（RSD） of intra-day was 4.35%.The method was simple,rapid and sensitive.

关 键 词：同型半胱氨酸 高效液相色谱法 库仑阵列电化学 血浆 

分 类 号：R318[医药卫生—生物医学工程]