丽格海棠再生体系的建立  

Establishment of Regeneration System of Rieger begonia

作　　者：张瑞越[1] 拾冲[1] 

机构地区：[1]淮阴师范学院生命科学学院,江苏淮安223300

出　　处：《安徽农业科学》2010年第32期18080-18081,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究丽格海棠组织培养再生植株的最佳培养基配方条件,为其快速繁殖提供技术参考。[方法]以丽格海棠幼嫩叶片为外植体,对其进行诱导、增殖和生根培养,确定其再生体系。[结果]以1 cm2左右带叶脉的幼嫩叶片为外植体,诱导愈伤和丛生芽的培养基为MS+6-BA 0.50 mg/L+NAA 0.50 mg/L+4%芦荟原汁,诱导率分别为94.44%和66.67%;生根培养基为1/2MS+NAA 0.10 mg/L,生根率为100%。[结论]该研究建立了丽格海棠离体再生体系。[Objective] The aim was to study the best medium formula for the tissue culture of regenerated plants of Rieger begonia so as to provide technical references for its rapid propagation.[Method] With the young leaves of Rieger Begonia as explants,which were induced,proliferated and rooting cultured in order to determine its regeneration system.[Result] With the Rieger begonia leaf of about 1 cm2 with main vein as explants,the suitable medium for callus and adventitious shoot induction was MS＋6-BA 0.50 mg/L＋NAA 0.50 mg/L＋4% aloe juice,and the induction rate were 94.44% and 66.67% respectively;the suitable medium for rooting was 1/2MS＋NAA 0.10 mg/ L with the rooting rate of 100%.[Conclusion] The study established regeneration system of Rieger begonia.

关 键 词：丽格海棠 组织培养 再生体系 

分 类 号：S685.99[农业科学—观赏园艺]