嗅鞘细胞移植到损伤脊髓存活时间的研究  

Survival Time of Olfactory Ensheathing Cells Transplanted into Injured Spinal Cord of Rat

作　　者：陈莉发[1] 段朝霞[2] 张洁元[2] 李兵仓[2] 余华荣[1] 

机构地区：[1]重庆医科大学基础医学院生理教研室,重庆400016 [2]第三军医大学大坪医院野战外科研究所第六研究室,创伤,烧伤与复合伤国家重点实验室,重庆400042

出　　处：《成都医学院学报》2010年第3期190-192,共3页Journal of Chengdu Medical College

基　　金：国家重点实验室Ⅰ类研究课题(SKLZZ200803)

摘　　要：目的观察植入损伤脊髓的自发绿色荧光的嗅鞘细胞(green fluorescent protein-olfactory ensheathing cells,GFP-OECs),研究移植后嗅鞘细胞的存活时间。方法用酶消化法分离培养来自2.5月龄的绿色荧光蛋白转基因大鼠嗅球最外层的嗅鞘细胞,培养第10天,用显微外科技术将嗅鞘细胞移植到脊髓挫伤后的T10节段,对照组移植术后未作特殊处理,实验组移植术后腹腔注射环孢菌素A,两组分别在移植术后1、2、4、8周取材,观察细胞植入损伤脊髓后存活情况。结果对照组在移植术后2周内可见存活的嗅鞘细胞;实验组在移植术后4周仍可见存活的嗅鞘细胞,8周未见存活的嗅鞘细胞。结论嗅鞘细胞移植后存活时间小于8周。Objective To observe the green fluorescent protein-olfactory ensheathing cells（GFP-OECs）,and study the survival of olfactory ensheathing cells.Methods Outermost olfactory ensheathing cells were isolated from the olfactory bulbs of adult green fluorescent protein transgenic rats,OECs were cultured by enzyme digestion.On the 10th day of culture in the medium,the cells were transplanted into contusion area of the 10th thoracic segment of spinal cord in SD rat with microsurgical technique.After transplantation,cyclosporin was injected intraperitoneally to the rats in the experimental group,and the rats in the control group did not receive any treatment.The GFP-OECs in the injured spinal cord were observed with fluorescent light microscopes after 1 week,2 weeks,4 weeks,and 8 weeks.Their survival was observed.Results Strong green fluorescence staining of GFP-OECs were observed in the control rats after 2 weeks’ transplantation.In experimental rats,strong green fluorescence staining of GFP-OECs were observed even after 4 weeks’ transplantation,but were not observed in 8 weeks’ translantation.Conclusion Transplant OECs cannot survive beyond 8 weeks.

关 键 词：嗅鞘细胞 GFP 脊髓损伤 存活 

分 类 号：R651.21[医药卫生—外科学]