大鼠小肠缺血/再灌注休克中一氧化氮、内皮素检测及意义  

Effects of Nitric Oxide and Endothelins in Intestinal Ischemic/Reperfusion Injury in Rat

作　　者：李湘楚[1,2] 何小飞[1,2] 熊建光[1,2] 于皆平[1,2] 

机构地区：[1]咸宁医学院附属第一医院内科 [2]湖北省人民医院消化内科

出　　处：《咸宁医学院学报》1999年第1期1-4,共4页Journal of Xianning Medical College

摘　　要：目的：观察一氧化氮（ＮＯ）对大鼠小肠缺血／再灌注（Ｉ／Ｒ）的作用及内皮素的改变。方法：复制内脏血管阻塞（ＳＡＯ）性休克模型，用左旋精氨酸（Ｌ－Ａｒｇ）及硝基精氨酸甲脂（Ｌ－ＮＡＭＥ）处理动物模型后分别测定血浆中ＥＴｓ、ＭＤＡ、组织蛋白酶Ｄ（ＣＤ）、ＮＯ－２／ＮＯ－３含量。结果：Ｌ－Ａｒｇ减缓了大鼠Ｉ／Ｒ血压下降（Ｐ＜０．０１），降低了血浆ＭＰＯ、ＬＤＨ、ＣＤ、ＭＤＡ的含量（Ｐ＜０．０１）及肠组织中伊文思蓝（ＥＢ）的含量（Ｐ＜０．０５）；Ｌ－ＮＡＭＥ与Ｌ－Ａｒｇ相反。ＭＰＯ与ＥＢ正相关（Ｐ＜０．０１），ＮＯ－２／ＮＯ－３与ＥＴｓ负相关（Ｐ＜０．０５）。结论：ＮＯ对小肠Ｉ／Ｒ损伤有保护作用，ＥＴｓ参与ＳＡＯ休克过程。Objective To observe the effects of nitric oxide(NO) on intestinal damage due to ischemic/reperfusion in rats and the changes of endothelins(ETs).Methods The model of splanic artery occulation(SAO) shock were made on the Wister rats. The contents of ETs, malondialehyde(MDA), cathepsin D(CD) and NO - 2/NO - 3were determinated in plasma after treatment with L-arginine(L-Arg) and L NAME the substrate for NO synthase, or an inhibitor of NO synthase.Results L-Arg attenuated the decrease of systemic blood pressure(P<0.01)after I/R, and decreased the contents of MDA, CD, ETs (P<0.01)in plasma and the content of Even’s blue dye(P<0.05) in intestinal tissuse as compared with I/R group. However,L-NAME had the opposite effects. In addition there were significantly positive correlation between MPO and EB (P<0.01) and inversely correlation between NO - 2/NO - 3 and ETs (P<0.05).Conclusions NO protected the intestine from I/R injury and attenuated the decrease of systemic blood pressure. It was possible that ETs is involved the mechanism of shock.

关 键 词：一氧化氮 内皮素 休克 小肠缺血 再灌注损伤 

分 类 号：R515.302[医药卫生—内科学]