基于RAPD技术的异爪蝗属两种蝗虫遗传关系研究  

Genetic Relationship of Two Euchorthippus Species using RAPD primers

作　　者：党莉[1] 郑先云[2] 郭亚平[3] 马恩波[4] 

机构地区：[1]山西生物应用职业技术学院,山西太原030031 [2]山西大学体育学院,山西太原030006 [3]山西大学生命科学学院,山西太原030006 [4]山西大学应用生物学研究所,山西太原030006

出　　处：《山西大学学报（自然科学版）》2010年第4期596-600,共5页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(3081010390730870302)

摘　　要：采用随机扩增多态性DNA(RAPD)技术对素色异爪蝗(Euchorthippus unicoloe(Ikonn))和邱氏异爪蝗(Euchorthippus cheui Hsia)共29个个体进行研究.10条随机引物共产生107条清晰、稳定的谱带.Ne i's基因多样性指数对RAPD数据分析表明:种间遗传分化系数为0.383 9,即38.39%的遗传分化存在于种间,61.61%的遗传多样性存在于种内.用UPGMA和NJ法对所有个体进行聚类分析,聚类结果显示:素色异爪蝗和邱氏异爪蝗同一种内个体首先相聚,然后两个种再相聚.The genetic divergence of two natural species of Euchorthippus unicoloe （Ikonn） and Euchor-thippus cheui Hsia was studied with RAPD method. 107 bands of 29 individuals revealed on the electro-thippus cheui Hsia was studied with RAPD method. 107 bands of 29 individuals revealed on the electro- pherogram amplified by 10 primers. Nei＇s index and a genetic distance matrix were analyzed. The results showed genetic differentiation coefficient estimated by Nei＇s index was 0. 383 9,that was,61.61% of the variation occurred within species and 38. 390% of the variation existed among species. The dendrograms based on Nei＇s genetic distance of RAPD markers were constructed using NJ and UPGMA indicating high similarities within species. The results achieved by the method of RAPD revealed the genetic pattern of variation. It is suggested that RAPD analysis is useful as molecular marker for grasshoppers in phylogenetic reconstruction at the species level.

关 键 词：异爪蝗 RAPD 遗传分化 

分 类 号：Q75[生物学—分子生物学] Q963