应用微生物发光法检测水体中的砷、镉、铬污染  

Apply Bioluminescence of Luminous Bacteria to Detect the Heavy Metal Contamination in Water

作　　者：吕品[1] 吴丽华[1] 仪慧兰[1] 

机构地区：[1]山西大学生命科学学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2010年第4期605-608,共4页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省工业攻关项目;太原市明星计划项目

摘　　要：采用细菌发光抑制实验,研究水体中As3+、Cd2+、Cr6+3种重金属的单一毒性和联合毒性.研究发现,在一定剂量范围内,As3+、Cd2+、Cr6+浓度与相对发光强度呈负相关;根据处理液对细菌发光强度的抑制效应,3种重金属的毒性大小依次为As3+>Cr6+>Cd2+,2种或3种重金属的联合毒性明显大于单一毒性,表现为相加或协同作用.研究结果表明,在一定浓度范围内,细菌发光强度的变化与重金属浓度具有剂量-效应关系,可利用发光细菌对水体重金属污染进行监测.Assessment of the single and joint toxicity effects of arsenic, cadmium and chromium was conducted by using biolumineseence inhibition test. The toxic substances can inhibit the bioluminescence of luminous bacteria,and then the relative luminous intensity could be used to judge the toxic effect. The experi- ment results showed that heavy metal ions inhibited bioluminescenee dose--dependently within a certain concentration range, according to the strength of bioluminescence inhibition, the list of the toxicity sequence was：As3＋〉Crs＋ 〉Cd2＋. The mixtures of two heavy metal ions As3＋ and Cr6＋ ,As3＋ and Cd2＋ ,and Cr6＋ and Cd2＋ , showed strong toxic activity comparing with their individual treatments, in which the joint toxicity effect was addictive or antagonism. The results suggested that luminescent bacteria could be used to monitor heavy metals contamination in water.

关 键 词：细菌发光抑制 重金属 水污染 毒性评价 

分 类 号：X832[环境科学与工程—环境工程]