IL-21在慢性乙型肝炎中免疫调节作用的研究  

STUDY ON IMMUNE REGULATION OF IL-21 IN CHRONIC TYPE B HEPATITIS

作　　者：马伟平[1] 何大馨[2] 钟丹丹[1] 张蓓[1] 

机构地区：[1]青岛大学医学院,山东青岛266021 [2]青岛市市立医院

出　　处：《青岛大学医学院学报》2010年第6期479-481,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省科技发展项目(2009GG10002044);青岛市科技计划项目(07-2-1-15-NSH)

摘　　要：目的 探讨白细胞介素21（IL-21）在慢性乙型肝炎中的免疫调节作用.方法 采用酶联免疫吸附试验（ELISA）检测32例慢性乙型肝炎病人（病人组）和29例健康人（对照组）血清中IL-21水平.提取外周血单个核细胞后分别设为IL-21刺激组、IL-2刺激组和未刺激组,多色流式分析技术检测CD8＋T细胞频率,胞内细胞因子染色技术检测CD8＋IFN-γ＋T细胞的频率.结果 与对照组相比,病人组血清中IL-21浓度明显增高（t=2.75,P＜0.01）;与IL-2刺激组和未刺激组相比,IL-21刺激组CD8＋T细胞频率及分泌IFN-γ水平显著升高（F=59.27、10.27,q=3.82～14.11,P＜0.01）.结论 IL-21通过对CD8＋T细胞的免疫调节效应参与慢性乙型肝炎病人体内病毒的清除,为临床治疗提供了新的靶标.Objective To investigate the role of IL 21 in patients with chronic hepatitis B （CHB）. Methods Serum IL- 21 in 32 patients with CHB and 29 healthy subjects were determined using enzymeqinked immunosorbent assay （ELISA）. After extraction of peripheral blood mononuclear cell, a setting of IL-21-stimulus, IL-2-stimulus and not-yet stimulus group was made. HBV DNA was analyzed with RT-PCR. The frequency of CD8＋ T cells was analyzed by flow cytometry, and that of IFN-γ by in- tracellular eytokine straining. Results Compared with the controls, the serum concentration of IL-21 in CHB was markedly higher （t=2.75,P〈0.01）; compared with IL-2 stimulus and not-yet stimulus, the frequency of CD8＋ T cells and secretion of CD8＋IFN-γ＋ T cells dramatically increased （F=59.27,10.27;q=3.82-14. 11;P〈0.01）. Conclusion IL-21 involves in eli- mination of viruses in the body of patients with CHB through immunological regulation of CD8＋ T cells, which provides a new tar- get for clinical therapy of the disease.

关 键 词：白细胞介素21 肝炎 乙型 慢性 CD8+T细胞 免疫调节 

分 类 号：R512.62[医药卫生—内科学]