树莓组织培养技术的研究  

Study on the Tissue Culture Technique in Raspberry

作　　者：曹慧[1] 薛佳桢[1] 孙京波[1] 

机构地区：[1]潍坊学院生物工程学院,山东潍坊261061

出　　处：《北方园艺》2010年第22期125-127,共3页Northern Horticulture

基　　金：潍坊学院试验资助项目;潍坊学院青年科研基金资助项目(2009Z19)

摘　　要：以树莓带芽茎段为外植体,摸索树莓组织培养快速繁殖技术体系,着重研究各阶段的最佳培养基配方及培养程序。结果表明:树莓组培外植体最佳取材时间应在5月初,半木质化的带芽茎段为其理想的外植体材料,外植体灭菌用75%乙醇0.5 min2、%NaClO 15 min效果最好;分化增殖最佳培养基为MS+6-BA 1.0 mg/L+NAA 0.02 mg/L+GA36 mg/L;最佳生根培养基为1/2MS+NAA 2.0 mg/L+IBA 1.0 mg/L+活性炭0.5%;组培苗移栽基质以有机肥∶土∶蛭石=1∶1∶1时成活率最高。The objective of this research was to study the tissue culture system of raspberry through explants of raspberry stem segment with buds,especially on optimum formula of culture medium and culture procedure at each phase.The results showed that the optional time of cutting explant was in early May,that the best explant was semi-lignified stem segment with buds,disinfected by 75% alcohol for 0.5 min and 2% NaClO for 15 min,that the optimal differentiation and rooting medium were MS＋6-BA 1.0 mg/L＋NAA 0.02 mg/L＋GA3 6 mg/L and 1/2MS＋NAA 2.0 mg/L＋IBA 1.0 mg/L＋activated carbon 0.05% respectively,that the cultured plantlets at the highest survival rate was transplanted in organic fertilizer,earth and vermiculite（1∶1∶1）.

关 键 词：树莓 组织培养 快速繁殖 

分 类 号：Q949.751.8[生物学—植物学]