稳心颗粒对大鼠心室肌细胞瞬时外向钾电流的影响  

Effects of Wenxin Granule on Transient Outward Potassium Current in Acute Isolated Ventricular Myocytes of Adult Rats

作　　者：王晞[1] 王鑫[1] 唐艳红[1] 王腾[1] 徐超[2] 黄从新[1] 

机构地区：[1]武汉大学人民医院心内科,430060 [2]武汉科技大学附属医院心内科,430064

出　　处：《医药导报》2010年第12期1539-1542,共4页Herald of Medicine

基　　金：湖北省"十一五"科技攻关项目(基金编号:2006AA301A04);湖北省自然科学基金资助项目(基金编号:2009CDB426)

摘　　要：目的研究稳心颗粒对大鼠心室肌细胞瞬时外向钾电流(Ito)的影响。方法采用全细胞膜片钳技术,观察不同浓度的稳心颗粒溶液对急性分离的成年大鼠心室肌细胞Ito动力学特性的影响。结果 1,5,10,20g.L-1稳心颗粒溶液可浓度依赖性地阻断Ito,对其峰电流的抑制率分别为(15.31±7.21)%,(32.86±5.08)%,(53.25±4.74)%,(73.23±4.11)%。各浓度稳心颗粒溶液均使Ito的I-V曲线下移,失活曲线右移。结论稳心颗粒对大鼠心室肌细胞Ito具有显著的抑制作用。Objective To study the effects of wenxin granule on the activation kinetics of transient outward potassium current（Ito）in single ventricular myocytes.Methods The whole-cell patch-clamp technique was used to investigate the effcts of wenxin granule（1,5,10,20 g·L-1）on Ito in acute isolated ventricular myocytes of adult rats.Results Wenxin granule inhibited the current density of Ito and the decreased rates of peak Ito were（15.31±7.21）%,（32.86±5.08）%,（53.25±4.74）%,（73.23%±4.11）% at 1,5,10,20 g·L-1,respectively.The current-voltage curve was shifted downward,and steady-state inactivation curve shifted rightward by wenxin granule.Conclusion Wenxin granule has antiarrhythmic effects by blocking ventricular myocytes Ito of rats.

关 键 词：稳心颗粒 全细胞膜片钳 钾电流 外向 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]