C5-siRNA对乳鼠心肌细胞缺氧复氧损伤的保护作用  

Protective Effect of C5-siRNA on hypoxia/reoxygenation injury in neonatal rat cardiomycytes

作　　者：何志红 吴素华[2] 苏德华[3] 唐 成伶俐 

机构地区：[1]盐步医院心内科,广东佛山528247 [2]中山大学附属第一医院心内科 [3]赣南师范学院体育学院

出　　处：《中国实验诊断学》2010年第11期1704-1706,共3页Chinese Journal of Laboratory Diagnosis

基　　金：广东省自然科学基金(06021338)

摘　　要：目的研究C5-siRNA对乳鼠心肌细胞缺氧复氧损伤的保护作用。方法用原代单层培养的心肌细胞分为4组:正常对照组(C组)、H/R组、H/R+C5-siRNA组、H/R+空载体病毒组。其中后三组均经历2小时缺氧和4小时复氧,H/R+C5-siRNA组及H/R+空载体病毒组在缺氧前2小时分别给予C5-siRNA及空载体病毒。用CKK-8快速比色法检测心肌细胞存活情况(OD值)及用Western Blot法及RT-PCR法检测C5。结果心肌细胞存活情况H/R+C5-siR-NA组显著高于H/R组及H/R+空载体病毒组,而C5表达量H/R+C5-siRNA组显著低于H/R组及H/R+空载体病毒组。结论 C5-siRNA对乳鼠心肌细胞缺氧复氧损伤具有保护作用,其机理可能与降低C5表达有关。Objective to study the protective effect of C5-siRNA on hypoxia/reoxygenation injury in neonatal rat cardiomycytes.Methods the cultured monolayer cardiomycytes of the first generation were randomly devided into four groups： the control group didnt receive any treatment,while the other groups including H/R group,H/R＋C5-sinRNA group,H/R＋empty vector-virus group all received 2-hour hypoxia and 4-hour reoxygenation.The H/R＋C5-siRNA group and H/R＋empty vector-virus group were subjected to C5-siRNA and empty vector virus in 2 hours before hypoxia.The cellular livability（the value of OD）was measured by CKK-8 colorimetry,and the expression of C5 were measured by RT-PCR and Wenstern Blot.Results the cellular livability of H/R＋C5-siRNA group was significantly higher than H/Rgroup and H/R＋empty vector-virus group,while the expression of C5 in H/R＋C5-siRNA group was lower than H/R group and H/R＋empty vector-virus group.Conclusion C5-siRNA could prevent hypoxia reoxygenation injury in neonatal rat cardiomycytes by reducing expression of C5.

关 键 词：C5-siRNA 缺氧-复氧损伤 RNA干扰 

分 类 号：R541[医药卫生—心血管疾病]