手术分娩对产妇和新生儿血中T淋巴细胞的影响  

Effect of operative delivery on T cell quantity in blood of parturients and newborns

作　　者：徐德朋[1] 陈复兴[2] 王全英[3] 周忠海[2] 颜芳[3] 

机构地区：[1]中国人民解放军第九七医院麻醉科,江苏徐州221004 [2]中国人民解放军第九七医院实验科,江苏徐州221004 [3]中国人民解放军第九七医院妇产科,江苏徐州221004

出　　处：《临床荟萃》2010年第24期2132-2134,共3页Clinical Focus

基　　金：南京军区医学科技创新课题基金资助(编号08MA039)

摘　　要：目的探讨手术分娩对产妇和新生儿T淋巴细胞亚群数量的影响。方法足月分娩产妇60例,依据分娩方式及手术时机分为自然分娩(NL)组、择期剖宫产(PCS)组、急诊剖宫产(ECS)组各20例;分别采集分娩时的产妇外周血和新生儿脐血,应用流式细胞仪检测T细胞各亚群的百分比。结果 NL组、PCS组、ECS组产妇血中T淋巴细胞亚群CD3+(69.15±13.24)%、(73.50±4.86)%、(68.68±7.50)%;CD3+CD4+(32.03±9.44)%、(36.35±11.82)%(、34.88±7.54)%;CD3+CD8+(29.83±6.01)%、(30.59±7.30)%、(28.08±4.09)%;CD4+/CD8+1.09±0.37、1.33±0.65、1.25±0.27各组间差异均无统计学意义(P>0.05);新生儿脐血:PCS组中由于CD4+的减少而呈现CD4+/CD8+的比值明显低于NL、ECS组,分别为2.71±0.86、3.52±1.50、3.30±1.03(P<0.05)。结论产妇血中T淋巴细胞亚群数量不受手术分娩以及产程影响;PCS可能会加剧新生儿对某些病原体的易感性。Objective To explore the effect of operative delivery on T cellular subsets quantity in blood of parturients and newborns.Methods Sixty term parturients based on delivery methods and operative time were divided into three groups（each group,n=20）,namely natural labor（NL）,periodic cesarean section（PCS）,and emergency cesarean section（ECS） group.The peripheral blood and cord blood were collected from parturients and newborns,respectively.The percentages of T cellular subsets in blood samples were detected by flow cytometry.Results T cell subsets CD3＋,CD3＋CD4＋,CD3＋CD8＋ and CD4＋/CD8＋,the data in NL,PCS and ECS group were CD3＋（69.15±13.24）%,（73.50±4.86）%,（68.68±7.50）%;CD3＋CD4＋（32.03±9.44）%,（36.35±11.82）%,（34.88±7.54）%;CD3＋CD8＋（29.83±6.01）%,（30.59±7.30）%,（28.08±4.09）%;CD4＋/CD8＋ 1.09±0.37,1.33±0.65,1.25±0.27.The percentage among three groups did not have significant difference in the parturient peripheral blood samples（P0.05）.Based on the low expressing levels of CD4＋ molecules,the newborn cord blood CD4＋/CD8＋ in PCS group was significantly lower than that of NL and ECS group,respectively 2.71±0.86,3.52±1.50,3.30±1.03（P0.05）.Conclusion Maternal blood T lymphocytes subsets quantity were not affected by the operative delivery and stages of labor.PCS may increase the susceptibilicy of newborn to some pathogens.

关 键 词：分娩 剖宫产术 免疫 T淋巴细胞亚群 新生儿 

分 类 号：R392.12[医药卫生—免疫学]