硝普钠分光光度法测定头孢噻肟钠  

Determination of cefotaxime sodium by spectrophotometry with sodium nitroprusside

作　　者：李晶[1] 詹艳艳[2] 杜新贞[2] 李全民[1] 

机构地区：[1]河南师范大学化学与环境科学学院,新乡453007 [2]西北师范大学化学与化工学院,兰州730070

出　　处：《分析试验室》2010年第12期25-27,共3页Chinese Journal of Analysis Laboratory

基　　金：河南省教育厅自然科学基金项目(2008B150011)资助

摘　　要：建立了测定头孢噻肟钠的新方法,碱性溶液中硝普钠与头孢噻肟钠生成了化学计量比为2∶1的产物,其最大吸收波长λmax=525 nm,表观摩尔吸光系数3.9×103L.mol-1.cm-1。头孢噻肟钠在0.91~200μg/mL浓度范围内与吸光度呈现良好线性关系,线性回归方程A=0.010＋0.00816ρ（μg/mL）,线性相关系数r=0.9994,检出限（3σ/k）为0.62μg/mL。方法可用于药物样品和血清中头孢噻肟钠含量的测定。A new method was proposed for the determination of cefotaxime sodium with sodium nitroprusside by spectrophotometry.Colored nitrosamine product was formed by cefotaxime sodium and sodium nitroprusside in an alkaline solution.The liner regression equation was A=0.010 ＋ 0.00816 ρ（μg/mL） with a correlation coefficient of 0.9994,and ε was 2.0×103 L·mol-1·cm-1 at 525nm.Beer＇s law is well obeyed in a range of 0.91～200 μg/mL over cefotaxime sodium concentration,the limit of detection（3σ/k） was 0.62 μg/mL,and RSD was 0.21%.The method was used for the determination of cefotaxime sodium in pharmaceutical samples and human serum with satisfactory results.

关 键 词：硝普钠 头孢噻肟钠 分光光度 

分 类 号：O657.3[理学—分析化学]