椭圆背角无齿蚌胃的组织学及铜对其结构的影响  

Histology of stomach of Anodonta woodiana elliptica and effect of heavy metal copper on its structure

作　　者：李雨奎[1] 

机构地区：[1]淮北师范大学

出　　处：《生态科学》2010年第5期461-466,共6页Ecological Science

摘　　要：硫酸铜常用于水产养殖的疾病防治,但当水体中的铜离子浓度超过一定范围时便对软体动物产生一定的毒害作用.研究选取椭圆背角无齿蚌（Anodonta woodiana elliptica）作为研究对象,采用苏木精-伊红染色方法进行组织石蜡切片制作,从组织学角度研究了浓度为0.01 mg·L- mg·L-1、0.1 mg·L-1、1 mg·L-1和10 mg·L-1的硫酸铜作用不同时间后椭圆背角无齿蚌的胃结构的变化.结果表明,0.01 mg·L-1硫酸铜处理7d和14d后,仅胃绒毛变短,随着硫酸铜浓度的增大和处理时间的延长,胃绒毛逐渐变短,肌层变薄,腺细胞数量减少直至空化;低于10 mg·L-1的硫酸铜中毒7d和14d后,胃晶杆头直径和胃楯长度出现增大的现象.CuSO4 is often used to prevent and cure disease in aquaculture, but is harmful to molluscs when its concentration is higher than a certain level. Histological changes of stomach ofAnodonta woodiana elliptica exposed to 0.01 mg·L-1, 0.1 mg·L-1, 1 mg·L-1 and 10 mg·L-1 of CuSO4 for different times were studied by making paraffin section stained with hematoxylin-eosin. The results showed that after A. woodiana elliptica was treated with 0.01 mg·L-1 CuSO4 for 7 and 14 days, cilium in stomach shortened. With the concentration increasing and the exposure time prolonging, cilium in stomach shortened significantly; muscular layer thinned; gland cells decreased and became eventually vacuoles. The diameter of style sac and the length of gastric shield increased when A. woodiana elliptica was exposed to CuSO4 concentrations lower than 10 mg·L-1 for 7 and 10 days, and the wall of ostium of A. woodiana elliptica thickened at CHSO4 concentration of 10 mg·L-1.

关 键 词：椭圆背角无齿蚌 组织学 硫酸铜 重金属 

分 类 号：Q178.532[生物学—水生生物学]