乳猪肝细胞的分离培养及其功能  

Isoation,culture and furction of pup pig hepatocytes

作　　者：刘树人 李灼亮 彭齐荣 余宙耀 张宜俊 

机构地区：[1]广东省广州市解放军458医院传染科,510602

出　　处：《世界华人消化杂志》1999年第5期375-377,共3页World Chinese Journal of Digestology

摘　　要：目的探讨乳猪肝细胞分离和培养的条件及其功能．方法用门静脉插管原位灌注结合胶原酶消化法分离乳猪肝细胞，应用条件培养基对其进行体外培养，观察其形态、增殖。分化和功能等方面的变化．结果发现乳猪肝细胞的得率为（７．２±２．２）×１０９／肝，活率为９５．０％±３．０％，体外培养的乳猪肝细胞，在长达９０ｄ的培养时间内能较好地维持其形态，原代和传代培养的乳猪肝细胞其ＤＮＡ和蛋白质的合成及ＡＬＴ和Ｇ—６—ＰＤ的活性相比，虽有变化，但趋势相同，两者无统计意义（Ｐ＞０．０５）．结论体外培养的乳猪肝细胞，在一定的培养条件下，能维持其生物学特性。AIM To study the isolation method and Culture conditionsof hepatocyte, and their morphology, proliferation andfunctions.METHODS POrtal vein cannulation and Per fusion combined with collagenase digestion were used to isolate pigpup hepatocytes. They were then cultured and passagedinto the conditioned medium. Their changes in morphology, proliferation and functions were studied by conventional methods.RESULTS The amount of hepatocytes harvested was (7 .2 2.2) x 109/ liver, and the viability of hepatocytes was95 .0% 3. 0%. In the Cultured Period of 90 days, hepato-cytes could better keep its morphology, proliferation andfunctions, as well as the enzyme activity (P >0. 05 ).CONCLUSION Under certain conditions, the pup pig pepa-tocytes cultured in Vitro could retain their morphologiccharacteristics, proliferative capacity and metabolic functions.

关 键 词：肝细胞学 培养细胞 功能 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R329-33[医药卫生—基础医学]