芹灵冲剂对雏鸭体内DHBV-DNA的抑制作用  

Inhibitory effect of Qinling granule on duck hepatitis B virus hepatitis model in nestling ducks in vivo

作　　者：黄正明[1] 杨新波[1] 曹文斌[1] 陈鸿雁 郑鸣[1] 张敬珍[1] 陈鸿珊[2] 李壮 

机构地区：[1]解放军北京医学高等专科学校 [2]中国医学科学院医药生物技术研究所病毒室,北京市100085

出　　处：《世界华人消化杂志》1999年第5期385-386,共2页World Chinese Journal of Digestology

基　　金：9.5军队重点科研基金

摘　　要：目的观察芹灵冲剂（ＱｉｎｌｉｎｇＣｈｏｎｇｊｉ，ＱＬ）对鸭体内鸭乙型肝炎病毒（ＤＨＢＶ）－ＤＮＡ的抑制效果方法待ＤＨＢＶ感染雏鸭７ｄ血清检测为阳性后，随机分组进行药物治疗试验，每组５只，ＱＬ分为高、中、低３个剂量组，分别为１０，５和２．５ｇ／ｋｇ组，ｉｇ，２次／ｄ，１０ｄ设病毒对照组（ＤＨ－ＢＶ），以生理盐水代替药物，ｉｇ，Ｂｉｄｘ１０．阳性药用无环鸟昔（ＡＣＶ）粉剂（２５ｍｇ／支），ｉｇ１００ｍｇ／ｋｇ，２次／ｄ，１０ｄ（Ｂｉｄｘ１０）在感染后７ｄ即用药前（Ｔ０），用药５ｄ（Ｔ５），用药１０ｄ（Ｔ１０）和停药后３ｄ（Ｐ３），自鸭腿经静脉取血，分离血清，－７０℃保存待检。结果ＱＬ（５，１０ｇ／ｋｇ）组，对雏鸭无毒性，给药５ｄ（Ｔ）和１０ｄ（Ｔ１０）能显著降低ＤＨＢＶ感染鸭血清中ＤＨＢＶ－ＤＮＡ的水平，其抑制率分别与病毒对照组比较，均有显著性差异（Ｐ＜０．０５和Ｐ＜０．０１）；低剂量组（２．５ｇ／ｋｇ）对鸭血清ＤＨＢＶ－ＤＮＡ也有一定的疗效３批实验结果一致，并有明显的量效关系结论ＱＬ体内有抗ＤＨＢＶ的作用，值得临床进一步研究．AIM To study the inhibitory effect of Qinling granule(QG) on duck hepatitis B virus (DHBV) hepatitis model innestling ducks in vivo.METHODS Seven-day--old DHBV infected nestling duckswere randomly divided into five groups: normal saline(NS); positive control drug, ACV 100 mg/ kg; QG10 g/ kg; QG 5g/ kg; and QG 2.5 g/ kg. Five animalswere used in each group. Drugs were administered orally,twice daily for 10 days. Blood was taken from leg vein andwere separated before treatment (TO), on day 5 and day10 (T5,T10) after treatment, and on day 3 (P3) after termination of treatment. The presence of DHBV DNA in serum was detected by dot-blot hybridization. Probe was labelled with 32 P. Livers were cut out for pathological examination.RESUI.TS In middle and high dosage groups of QG (5 and10 g/ kg), serum DHBV--DNA levels were significantly lowered (P<0. 05 and P< 0 .01 ), and the effect was similar inlow dose group.CONCLUSION Qingling granule can inhibit DHBV in vivo.It is worthy of further studies as an anti--hepatitis drug.

关 键 词：中医药疗法 芹灵冲剂 乙肝病毒 DNA 

分 类 号：R259.126.2[医药卫生—中西医结合]