地塞米松上调人胸膜间皮细胞水分子通道蛋白1基因的表达  

Dexamethasone induces overexpression of aquaporin 1 in human pleural mesothelial cell

作　　者：姜燕[1] 赵年贵[1] 陈玲玲[1] 吴学记[3] 玄先法[2] 王玉新[2] 

机构地区：[1]厦门市第二医院呼吸内科,361021 [2]厦门市第二医院肾内科,361021 [3]厦门大学生命科学学院生物医学系,361005

出　　处：《国际呼吸杂志》2010年第23期1435-1438,共4页International Journal of Respiration

摘　　要：目的研究地塞米松对人胸膜间皮细胞水分子通道蛋白1（AQP1）表达的影响。方法选择非感染性、良性胸腔积液患者，抽取胸水10m1，经离心分离、获取原代胸膜间皮细胞并进行体外培养，分别以0μmol／L、1μmol／L、100μmol／L浓度的地塞米松进行预处理后再继续进行培养，采用Western blotting及荧光定量PCR检测细胞AQP1蛋白与mRNA表达水平。结果从胸腔积液中分离与培养的人胸膜间皮细胞活细胞率和纯度均为95％以上；经地塞米松处理后的人胸膜间皮细胞AQP1与mRNA的表达水平显著增高，而且与地塞米松浓度呈正相关。结论地塞米松可以在转录水平和翻译水平上增强AQP1基因的表达，且有剂量依赖性特点。提示地塞米松可以对AQP1表达起上调作用，进而加速胸膜腔内液体的吸收。Objective To study the effect of dexamethasone on the expression of aquaporin 1 （AQP1）in pleural mesothelial cell patients with pleural fluid. Methods Human pleural mesothelial cell was obtained from the patients with benign and non infective pleural fluid and cultivated after preconditioning the first generation cell by 0 μmol/L, 1 μmol/L, 100μmol/L dexamethasone respectively, and the expression level of AQP1 protein and mRNA was detected by western blotting and RT-PCR separately. Results The survival rate and purity of human pleural mesothelial cell isolated and cultivated from pleural fluid are both above 95 %,the expression level of AQP1 protein and mRNA of human pleural mesothelial cell induced by dexamethasone are raised up significantly. The expression level has direct correlation with the density of dexamethasone. Conclusions Dexamethasone can up-regulate AQP1 gene expression dose-dependent at translational and transcriptional level. And then accelerate the absorption of hydrothorax.

关 键 词：人胸膜间皮细胞 水分子通道蛋白1 地塞米松 

分 类 号：R5[医药卫生—内科学]