甘蔗乙烯受体基因Sc-ERS的克隆与序列分析  

Cloning and sequencing of sugarcane ethylene receptor gene(Sc-ERS)

作　　者：魏源文[1] 邓智年[1] 黄诚梅[1] 潘有强[1] 吕维莉[1] 李杨瑞[1] 

机构地区：[1]广西作物遗传改良生物技术重点开放实验室,南宁530007

出　　处：《广西农业科学》2010年第10期1036-1040,共5页Guangxi Agricultural Sciences

基　　金：国家科技支撑计划项目(2007BAD30B02);广西科技基础条件平台建设项目(09-007-08);广西科学研究与技术开发计划项目(桂科能0815011-6-1);广西科学基金项目(桂科基0778006-6);广西科学基金项目应用基础研究专项项目(桂科基0991021);广西农业科学院科技发展基金项目(2007022)[e-mail]

摘　　要：根据已知乙烯受体基因的序列信息,设计1对简并引物和1对特异引物,对甘蔗叶片基因组DNA进行PCR扩增,获得1个甘蔗乙烯受体基因(Sc-ERS)的片段,其大小为4310 bp,包含1个由5个外显子和4个内含子组成的4219 bp大小的完整编码区。Sc-ERS编码区与玉米乙烯受体ERS1基因(AY359578)、水稻乙烯受体ERS1基因(AY043031)编码区的同源性分别为91.4%、61.6%;推导Sc-ERS编码的蛋白包含636个氨基酸残基,与玉米、水稻乙烯受体基因所编码蛋白的氨基酸同源性分别为94.1%、89.4%。其推导蛋白结构分析结果显示,Sc-ERS编码的蛋白和玉米、水稻ERS1所编码的蛋白相同,在N段保守区含有3个跨膜结构,并由3个跨膜结构、GAF结构、HisKA和HATPase_c组成功能域。核苷酸序列分析和蛋白质结构分析结果表明,Sc-ERS基因属于ERS1基因。A pair of degenerate primers and a pair of specific primers were designed based on the conserved sequences of plant ethylene receptor genes to amplify the genomic DNA of sugarcane.A 4310 bp fragment of sugarcane ethylene receptor gene（Sc-ERS） was cloned containing the entire coding region of 4219 bp,consisted of five extrons and four introns.The coding region of Sc-ERS showed 91.4 and 61.6% homology with maize ERS1（AY359578） and rice ERS1（AY043031）,respectively.The deduced protein of Sc-ERS encoded 636 amino acids,and shared 94.1 and 89.4 % similarity with maize and rice ERS1,respectively.The architecture of deduced protein analysis indicated that the Sc-ERS contained three trans-membrane structures at the conserved N end,similar to maize and rice ERS1.The functional region of the coding protein of Sc-ERS in sugarcane,ERS1 in maize and rice consisted of three trans-membrane structures,viz,GAF,HisKA and HATPase_c domain.These results indicated that the Sc-ERS in sugarcane was similar to the ERS1 of other plants.

关 键 词：甘蔗 乙烯受体基因 克隆 序列分析 

分 类 号：S566.1[农业科学—作物学]