豌豆MAR的分离及其功能分析  

Isolation of pea MAR sequence and its functional analysis

作　　者：邓智年[1] 魏源文[1] 黄诚梅[1] 潘有强[1] 吕维莉[1] 李杨瑞[1] 

机构地区：[1]广西作物遗传改良生物技术重点开放实验室,南宁530007

出　　处：《广西农业科学》2010年第10期1041-1045,共5页Guangxi Agricultural Sciences

基　　金：国家科技支撑计划项目(2007BAD30B02);广西科技基础条件平台建设项目(09-007-08);广西科学研究与技术开发计划项目(桂科能0815011-6-1;桂科攻10100005-9);广西科学基金项目(2010GXNSFA013102);广西科学基金项目应用基础研究专项(桂科基0991021);广西农业科学院科技发展基金项目(2007023)

摘　　要：从3个不同品种豌豆中分离获得3个具有MARs序列特征的DNA片段MMAR、DMAR、HMAR。根据GenBank同源性搜索结果,发现这些片段均为未注册的DNA序列。经转化烟草并测定其GUS活性,结果发现两侧顺式重复连接MAR对外源基因的表达具有明显的增强作用,MMAR、HMAR和DMAR均不同程度提高了GUS的表达,分别为载体对照植株的4.16、3.66和2.08倍,但不同转化体间表达水平差异仍比较明显。Three new MAR segments（MMAR,DMAR and HMAR） were cloned from three kinds of peas.These MAR segments were different from the original MAR sequences and unregistered in GenBank.In order to investigate the gene expression of these MARs in transgenic plants,the plant expression vectors were constructed with udiA gene coding β-glucuronidase（GUS） which was flanked on both sides by the MARs in the same direction.Quantitative detection of GUS acti-vity in transformed tobacco plants showed that the MARs could increase GUS expression level in vivo in contrast to the control.The average GUS activity of the MMAR,DMAR and HMAR was 4.16,3.66 and 2.08 times higher than the control pBI121,respectively.The expression differences among the individual transformants were also significant.

关 键 词：豌豆 核基质附着区(MARs) GUS表达 

分 类 号：S643.3[农业科学—蔬菜学]