广西黑山羊伪结核棒状杆菌的分离与鉴定  

Isolation and identification of Corynebacterium pseudotuberculosis from black goat in Guangxi

作　　者：郑敏[1] 胡杰[1] 欧绍毅 陈义祥[1] 李向涛[3] 李春艳[3] 陆文俊[1] 苏凯[1] 

机构地区：[1]广西动物疫病预防控制中心,南宁530001 [2]大新县动物疫病预防控制中心,广西大新532300 [3]广西大学动物科学技术学院,南宁530005

出　　处：《广西农业科学》2010年第10期1128-1130,共3页Guangxi Agricultural Sciences

基　　金：广西青年科学基金项目(桂科青0991042);广西科技创新能力与条件建设项目(08-05-01D)

摘　　要：从广西某养殖户发病黑山羊的脓肿脓汁中分离获得1株无荚膜、不形成芽孢的革兰氏阳性球杆菌,该菌在含血液或血清的琼脂培养基上,可生长成直径1 mm左右、干燥、中央凸起、不透明呈灰白色、边缘不整齐、伴有β溶血环的菌落,而在普通培养基上生长不良,在麦康凯琼脂培养基上不能生长。经生化试验、PCR鉴定及测序分析,证实该菌为伪结核棒状杆菌,动物试验证实该菌为致病菌。最后根据药敏试验结果选择敏感抗生素对发病黑山羊进行治疗,并提出综合防控措施。Using bacterioscopy,a non-capsulated,non-spore forming gram-positive spherical bacteria was found in abscess sampled from sick black goat from a village in Guangxi.The bacteria were found to produce about 1 mm diameter dry colony which was uplifted centrally,opaque,pale and jagged with narrow β hemolytic cells on agar plate containing blood or serum.However,it grew poorly on general agar medium,but,could not grow completely on Mac Conkey agar medium.Further,biochemical tests,PCR detection and sequence analysis confirmed this bacterium as a Corynebacterium pseudotuberculosis strain.Regression tests confirmed it as pathogenic bacterium.Finally,all sick goats were treated using sensitive antibiotics,and comprehensive prevention and control measures were undertaken.

关 键 词：伪结核棒状杆菌 黑山羊 分离 鉴定 广西 

分 类 号：S858.27[农业科学—临床兽医学]