生菜基因转化组织培养受体系统的建立  

Establishment of Receptor System of Gene Transformation of Lettuce

作　　者：罗雯[1] 孙东妮[1] 王甜[1] 王钢[1] 

机构地区：[1]西安文理学院生命科学系,西安710065

出　　处：《科学技术与工程》2010年第34期8384-8388,共5页Science Technology and Engineering

基　　金：国家自然科学基金(30471626)资助

摘　　要：以散叶生菜大速生(Lactuca sativavar.capatata L.)为试材,以MS为基本培养基,采用不同的激素配比,经愈伤组织诱导、芽分化、生根、移植入土四个步骤的离体培养,获得正常的再生植株,建立了散叶生菜大速生的基因转化受体系统,为下一步的基因转化工作提供了有利条件。高效诱导愈伤组织培养基为MS+0.5mg/L6-BA+0.1mg/L NAA,高效诱芽培养基为MS+0.1mg/L6-BA+0.05mg/L NAA。同时确定了子叶外植体对抗生素的敏感性。试验表明,筛选培养基中适宜的卡那霉素选择压在100mg/L以下,抑菌剂羧苄青霉素或头孢噻肟钠的适宜质量浓度均为300mg/L。Studies on establishment of receptor system of gene transformation of lettuce（Lactuca sativa var.capatata L.）were reported.The normal regenerated plants have been obtained from cotyledons of seedings by a three-step culture procedure——callus induction and shoot differentiation,rooting of excised shoot and transplanting into soil.MS was the basal medium in all steps,supplemented with different kind and different concentration of phytohormones.The results show that MS medium supplies with 0.5 mg/L 6—BA and 0.1 mg/L NAA is suitable for callus regeneration,while MS medium supplies with 0.1 mg/L 6—BA and 0.05 mg/L NAA is suitable for shoot regeneration.The experiments on sensitivity of cotyledon to antibiotics shows that the suitable kanamycin concentration for selecting transgenic tissue is no more than 100 mg/L,the carbenicillin or cefotaxime concentration is 300 mg/L.The receptor system of gene transformation of lettuce is then established.

关 键 词：生菜 基因转化 组织培养 

分 类 号：S636.2[农业科学—蔬菜学]