不同浓度瘦素对人胚胎绒毛滋养细胞膜胰岛素受体磷酸化的抑制作用  

Inhibitory effect of leptin on phosphorylation of insulin receptors in trophoblast cells

作　　者：李素芬[1] 杨鹰[1] 

机构地区：[1]第三军医大学新桥医院妇产科,重庆400037

出　　处：《第三军医大学学报》2010年第23期2530-2532,共3页Journal of Third Military Medical University

基　　金：重庆市自然科学基金面上项目(2007)~~

摘　　要：目的研究不同浓度瘦素对人胚胎绒毛滋养细胞胰岛素受体(InsR)酪氨酸磷酸化水平的影响,探讨瘦素在胰岛素受体信号转导分子机制中的作用。方法体外培养人工流产胚胎的绒毛滋养细胞,根据培养基中加入瘦素的浓度不同将实验分为空白对照组以及瘦素浓度分别为100、250、500μg/L的实验组。每组分别培养24h后,采用Western blot方法测定滋养细胞膜磷酸化胰岛素受体β亚基的含量。结果滋养细胞细胞膜磷酸化胰岛素受体β亚基水平测定:浓度分别为100、250、500μg/L的实验组胰岛素受体的磷酸化水平均显著低于对照组(P<0.05),并随着瘦素浓度的上升,绒毛滋养细胞细胞膜磷酸化胰岛素受体β亚基的含量进一步下降。空白对照组的磷酸化胰岛素受体蛋白水平为(1.2162±0.0322),当瘦素浓度分别为100、250μg/L和500μg/L时,磷酸化的胰岛素受体蛋白水平分别为(1.0567±0.0424)、(0.9576±0.0268)和(0.7342±0.0388)。瘦素浓度和滋养细胞膜胰岛素受体β亚基磷酸化水平呈负相关(r=-0.4032,P<0.05)。结论瘦素与胰岛素受体信号转导系统有相关性,抑制细胞膜胰岛素受体β亚基磷酸化可能是其影响胰岛素受体信号转导的机制之一。Objective To study the effect of leptin at different concentrations on phosphorylation of insulin receptors in human trophoblast cells and its role in molecular mechanism underlying insulin receptor signal transduction. Methods Trophoblast cells from artificially aborted embryo were cultured in vitro and divided into blank control group, 100 μg/L leptin group, 250 μg/L leptin group and 500 μg/L leptin group. After incubated for 24 h, phosphorylation of insulin receptors was detected by Western blotting. Results The phosphorylation of insulin receptor β subunit in trophoblast cells was significantly lower in 100 μg/L leptin group, 250 μg/L leptin group and 500 μg/L leptin group than in control group （P0.05）, which decreased with the increasing concentration of leptin. The phosphorylation of insulin receptor protein in trophoblast cells was 1.216 2±0.032 2 in control group, and 1.056 7±0.042 4, 0.957 6±0.026 8, 0.734 2±0.038 8, respectively, in 100 μg/L leptin group, 250 μg/L leptin group, and 500 μg/L group. Conclusion Leptin is correlated with insulin receptor signal transduction. Inhibition of phosphorylation in cell membrane insulin receptor β subunit may be one of the mechanisms underlying insulin receptor signal transduction.

关 键 词：瘦素 胰岛素受体磷酸化 滋养细胞 胰岛素受体信号转导 

分 类 号：R321.5[医药卫生—人体解剖和组织胚胎学] R394.2[医药卫生—基础医学]