中国百日咳鲍特菌ptxS1和prn基因多态性分析  

Polymorphism of ptxS1 and prn Genes of Bordetella pertussis in China

作　　者：张柳[1,2] 徐颖华[1] 赵建宏[2] 徐运强[3] 张庶民[1] 

机构地区：[1]中国药品生物制品检定所血清室,北京100050 [2]河北医科大学第二医院,石家庄050000 [3]河北省石家庄市第二医院检验科,石家庄050000

出　　处：《中国生物制品学杂志》2010年第11期1158-1162,共5页Chinese Journal of Biologicals

摘　　要：目的分析我国近50年来分离的百日咳鲍特菌抗原百日咳毒素(Pertussistoxin,PT)S1亚基(ptxS1)和百日咳黏附素(Pertactin,prn)基因多态性。方法收集85株百日咳杆菌,分别进行ptxS1和prn基因的PCR扩增和测序,并对序列进行比较分析。结果共发现4个ptxS1基因型和6个prn基因型的百日咳杆菌。自20世纪60年代,非疫苗型ptxS1A菌株逐渐取代疫苗型菌株;含有prn2和prn3新基因型的菌株出现在2000年以后。基因的系统进化树显示,菌株ptxS1和prn基因型的核苷酸和氨基酸序列的同源性分别在97%以上。结论我国近50年流行的百日咳临床分离菌株ptxS1和prn基因存在抗原漂移现象,本研究为加强我国百日咳的流行病学监控与新型无细胞百日咳疫苗的研发奠定了基础。Objective To analyze the polymorphism of pertussis toxin（PT）S1 subunit（ptxS1）and pertactin（prn）genes of Bordetella pertussis in China in the past 50 years.Methods A total of 85 B.pertussis strains were collected,from which ptxS1 and prn genes were amplified by PCR and sequenced,and the sequencing results were compared.Results The B.pertussis of four ptxS1 genotypes and six prn genotypes were found.Since 1960s,non-vaccine-derived ptxS1A strains have gradually substituted to the vaccine-derived ones.The strains containing novel prn2 and prn3 genotypes appeared after 2000.Phylogenetic tree showed that both the homologies of nucleotide and amino acid sequences of strains of ptxS1 and prn genotypes were more than 97%.Conclusion Antigen drift was observed in the ptxS1 and prn genes of clinical isolates of B.pertussis in China during the past 50 years.It laid a foundation of strengthening the epidemiological monitoring of pertussis in China and developing novel acellular pertussis vaccine.

关 键 词：百日咳鲍特菌 临床分离株 基因 序列分析 

分 类 号：R378.42[医药卫生—病原生物学] Q78[医药卫生—基础医学]